Protease

Details Protease Protease

1993-03-23

1995-10-17

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352523, 4353201, 536 232, C12N 1511, C12N 1552

Patent

active

054590647

ABSTRACT:
A novel protease derived from Bacillus licheniformis is provided. The protease cleaves the peptide bonds at the carboxyl termini of glutamic acid residues in polypeptides. The protease contains an amino acid sequence from serine in the +1 position to glutamine in the +222 position of SEQ ID NO: 1.

REFERENCES:
patent: 4266031 (1981-05-01), Tang
Okada, J., et al., "Genetically engineered Bacillus subtilis for extracellular protease manufacture," Chem Abstracts (1986) 104(19): 164 (Abstract No. 163023). A copy of the corresponding Japanese Patent 61-12287 is also enclosed herewith.
Drapeau, G. R., et al., "Purification and Properties of an Extracellular Protease of Staphylococcus aureaus", J. Biol. Chem. (1972) 247:6720-6726.
Carmona, C., et al., "Nucleotide Sequence of the Serine Protease Gene of Staphylococcus aureus, Strain V8", Nucl. Acids Res. (1987) 15(16):6757.
Yoshida, N., et al., "Purification and Characterization of an Acidic Amino Acid Specific Endopeptidase of Streptomyces griseus Obtained from a Commercial Preparation (Pronase)", J. Biochem. (1988) 104(3):451-456.
Niidome, T., et al., "Purification and Characterization of an Acidic Amino Acid-Specific Endopeptidase of Bacillus subtilis Obtained from a Commercial Preparation (Protease Type XVI, Sigma)", J. Biochem. (1990) 108:965-970.
Stahl et al., "Confirmation of Protoplast Fusion-Derived Linkages in Staphylococcus aureus by Transformation with Protoplast DNA", J. Bacteriol, (1983) 154(1):406-412.
Stahl et al., "Replacement of the Bacillus subtilis Subtilisin Structural Gene with an In Vitro-Derived Deletion Mutation", J. Bacteriol. (1984) 158(2):411-418.
Vitkovic, L., et al., "Purification of the Extracellular Protease of Bacillus licheniformis and Its Inhibition by Bacitracin", J. Bacteriol. (1977) 131(3):891-896.
Akparov, V., et al., "Extracellular Serine Proteinase from Bacillus licheniformis", Chemical Abstracts (1983) 98(9), p. 252, Abstract No. 98:67639g.
European Patent Application Sequence Listing Abstract R14159 A-Gene Sequence 7. (This publication was cited by Examiner Schmikel with Paper #10 for the related application, Ser. No. 07/782,372).
Suggs et al Proc Natl. Acad Sci USA 78 No. 11. pp. 6613-6617 Nov 1981.

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