Propylene glycol as an activator for phosphoenolpyruvate carboxy

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 14, 435 15, 435 18, 435183, C12Q 100, C12Q 154, C12Q 148, C12Q 134

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active

053044690

ABSTRACT:
The present invention provides an improved method and reagent for measuring the total carbon dioxide content in a sample of body fluid. The improvement comprises adding a sufficient amount of propylene glycol to the PEPC enzyme reagent to increase the activity of PEPC and maintaining the enzyme reagent at a substantially constant pH of about 6.0 to 7.0.

REFERENCES:
patent: 3963578 (1976-06-01), Aitken et al.
patent: 3974037 (1976-08-01), Adams
patent: 4250254 (1981-02-01), Modrovich
patent: 4704365 (1987-11-01), Yost
"Reagents for Enzymatic Analysis" in Methods of Enzymatic Analysis, 3rd ed. Bergmeyer [Weinheim: Verlag Chemic, 1983].
"Carbon Dioxide and Bicarbonate" in Manometric & Biochemical Techniques, ed. Umbreit [Minneapolis: Buress Publishing Co., 1972].
Waygood et al., Can. J. Bot, (1969) 47:1455-58.

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