Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1989-10-17
1994-04-19
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 14, 435 15, 435 18, 435183, C12Q 100, C12Q 154, C12Q 148, C12Q 134
Patent
active
053044690
ABSTRACT:
The present invention provides an improved method and reagent for measuring the total carbon dioxide content in a sample of body fluid. The improvement comprises adding a sufficient amount of propylene glycol to the PEPC enzyme reagent to increase the activity of PEPC and maintaining the enzyme reagent at a substantially constant pH of about 6.0 to 7.0.
REFERENCES:
patent: 3963578 (1976-06-01), Aitken et al.
patent: 3974037 (1976-08-01), Adams
patent: 4250254 (1981-02-01), Modrovich
patent: 4704365 (1987-11-01), Yost
"Reagents for Enzymatic Analysis" in Methods of Enzymatic Analysis, 3rd ed. Bergmeyer [Weinheim: Verlag Chemic, 1983].
"Carbon Dioxide and Bicarbonate" in Manometric & Biochemical Techniques, ed. Umbreit [Minneapolis: Buress Publishing Co., 1972].
Waygood et al., Can. J. Bot, (1969) 47:1455-58.
Abbott Laboratories
Escallon Miguel H.
Schmidt Richard D.
Wax Robert A.
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