Propagation process employing agarose medium

Details Propagation process employing agarose medium Propagation process employing agarose medium

1988-06-03

1991-03-12

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352404, 43524048, 43524049, 43524054, 47DIG11, C12N 504

Patent

active

049992996

ABSTRACT:
The invention describes a process for culturing proliferating aggregates of plant cells, which comprises (a) uniformly plating isolated protoplasts, or isolated cells regenerated from protoplasts, in or on an agarose-solidified culture medium, and/or (b) cutting this pretreated and solidified culture medium into segments, transferring said segments to a liquid nutrient solution and continuing culturing in both cases until the cell aggregates have attained the desired size.

REFERENCES:
patent: 3514900 (1970-06-01), McDade
patent: 4217730 (1980-08-01), Abo El-Nil
patent: 4338745 (1982-07-01), Misawa et al.
patent: 4473648 (1984-09-01), Tang
Maramorosch et al; Practical Tissue Culture Applications, Academic Press, N.Y., 1979, pp. 31-34.
Reinert et al., Plant Cell and Tissue Culture; Springer-Verlag, N.Y., 1982, pp. 40-45.
Singleton et al., Dictionary of Microbiology; John Wiley & Sons, New York; .COPYRGT. 1978; p. 7.
Staba, E. John; Plant Tissue Culture as a Source of Biochemicals; CRC Press Inc., Boca Raton, Fla., .COPYRGT. 1980; pp. 100-102.

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