Propagation of babesia parasites

Details Propagation of babesia parasites Propagation of babesia parasites

1980-03-31

1981-12-22

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435258, 435947, C12Q 118

Patent

active

043071910

ABSTRACT:
A method for quantitative propagation of hemotropic parasites, especially Babesia parasites, in vitro, Babesia-infected erythrocyte cultures are incubated in an environment of enhanced carbon dioxide tension wherein the culture medium overlay provides a barrier to oxygen transmission such that cellular hemoglobin is essentially maintained in its deoxy state. By varying environmental carbon dioxide tension, merozoite stages of the parasite are selectively reversibly induced to migration between residence in host cells or culture medium, rendering possible the isolation of infective antigenic merozoites in large quantities. Specific Babesia antigen is harvested in large quantity from the in vitro culture medium.

REFERENCES:
patent: 3743579 (1973-07-01), Zilberblat
William Trager et al., Science, vol. 193, pp. 673-675; 1976.
Clarence A. Speer et al., Z. Parasitenk, vol. 50, pp. 237-244; 1976.
Elton E. Erp et al., Am. Four. Trop. Med. Hyg., vol. 27, No. 5, pp. 1061-1064; 1978.

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