Propagating multiple whole fertile plants from immature legumino

Details Propagating multiple whole fertile plants from immature legumino Propagating multiple whole fertile plants from immature legumino

1988-05-23

1991-04-16

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524048, 4352405, 43524054, C12N 500

Patent

active

050082000

ABSTRACT:
Seeds of Glycine max, Glycine soja and hybrids thereof are produced by propagating embryogenic tissue containing multiple immature somatic embryos directly or serially by culturing particular cotyledonary tissue or derivation product thereof on media containing auxin selected from the group consisting of chloro-substituted phenoxyacetic acid, methyl derivative thereof, dicamba or picloram, incubating individual immature somatic embryos or embryogenic tissue containing such in a maturation stage to produce mature somatic embryos, germinating the mature somatic embryos to cause shoot tip formation, cultivating the germinated embryos to provide plantlets with 2 to 5 nodes and roots, cultivating the plantlets to provide whole fertile plants which flower and bear seeds, and recovering the seeds. Culturing to provide immature somatic embryos is carried out utilizing auxin in a concentration within the range of 2.5 to 1000 ppm effective to foster normal development of immature somatic embryos. 2,4-dichlorophenoxyacetic acid is the preferred auxin. Maturation is carried out in B5 or MS medium containing indolebutyric acid and abscissic acid or activated charcoal and sucrose, and germination is carried out in B5 or MS medium containing indolebutyric acid and gibberellic acid or MS medium containing filter sterilized L-proline and sucrose. Plantlet formation is carried out in B5 or MS medium without growth regulators. Plant and seed formation is carried out in soil or potting medium.

REFERENCES:
patent: 4684612 (1987-08-01), Hemphill et al.
Lippmann et al., 1984, Plant Cell Rep. 3: 215-218.
Lazzeri et al., 1985, Plant Mol. Biol. Rep. 3(4): 160-167.
Hammatt et al., 1987, J. Plant Physiol. 128 (3): 219-226.
Ammirato, P. 1983, pp. 100-104 In: Handbook of Plant Cell Culture, vol. 1, Evans et al., eds., MacMillan-New York.

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