Proofreading primer extension

Details Proofreading primer extension Proofreading primer extension

2011-08-02

2011-08-02

Babic, Christopher M. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07989168

ABSTRACT:
The present invention provides for primer extension reactions, including polymerase chain reactions, in which a polymerase having 3′-5′ exonuclease activity edits a primer that is not fully complementary thereby allowing for amplification and detection of target nucleic acids that may have variability in their sequences.

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Fujiwara, Hiroshi, et al, 1994, “PCR with Deoxyinosine-containing Primers Using DNA Polymerases with Proofreading Activitiy”, PCR Methods and Applications, 239-240.

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