Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Patent
1995-05-19
1997-08-19
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
435325, 514 2, 530350, 530827, 530828, 530834, C12N 500, C12N 508, C07K 1400
Patent
active
056587899
ABSTRACT:
A method for growing epithelial cells in vitro using soluble proteins secreted by 804G and NBT-II rat bladder carcinoma cells. These proteins stimulate cell attachment and hemidesmosome formation in cells grown in contact with the proteins. The protein is purified from conditioned medium by immunoaffinity chromatography using a monoclonal antibody generated against the protein. The purification of these proteins may be facilitated by culturing the cells under low serum or serum free conditions.
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Hormia Marketta
Quaranta Vito
Desmos, Inc.
Larson Kristin K.
Wityshyn Michael G.
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