Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1985-12-05
1991-02-05
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4353201, 435255, 435200, 935 41, 935 37, 935 48, 536 27, C12N 1500, C12N 116, C12N 924, C07H 1512, C12P 2102
Patent
active
049904469
ABSTRACT:
Promoters capable of ensuring expression in yeast of genes coding for heterologous polypeptides are disclosed, which comprise a DNA fragment or mutants or sub-fragments thereof, said promoter comprising a definite nucleotide sequence commencing with an EcoRI site and terminated by another restriction site. Also disclosed are vector plasmids containing the promoters, transformed yeasts comprising these plasmids, and a process for preparing polypeptides and especially a 1,4-.beta.-N-acetylmuramidase.
REFERENCES:
Dobson et al., Conservation of High Efficicacy Promotor Sequences in Saccharomyces Cerevisiae NAR (1982), 10:8, 2625-2637.
Hitzman et al, Secretion of Human Interferons by Yeast Science (1983), 219, 620-624.
Guarantc et al., Improved Methods for Maximizing Expression of a Coned Gene: a Bacterium that Synthesis Rabbit B Globin Cell 1980 20:543-553.
Rothstein et al., Secretion of a Wheat & Amylase Expressed in Yeast, Nature (1984), 308:12, 662-665.
Davison John R. N.
Oberto Jacques
Abokhair John K.
Carson Patricia
Cheairs M. Norwood
Labofina S.A.
Parkhurst Roger W.
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