Promoters for gene expression

Details Promoters for gene expression Promoters for gene expression

1995-05-19

1997-05-13

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Vector, per se

536 231, 536 2351, 536 241, C12N 1511, C12N 1516, C12N 1563

Patent

active

056292058

ABSTRACT:
The present invention provides a novel expression system for the production of protein in bacterial hosts. The system utilizes novel promoters that are highly efficient in initializing transcription and therefore enhance protein yield. The promoters comprise the -35 region of the consensus E. coli promoter, the -10 region of the lppP-5 promoter and a spacer between these two regions derived from either the lpp or lacUV5 promoters.

REFERENCES:
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Nicolas Groch, et al., "Synthesis of the Bacillus subtilis histone-like DNA-binding protein Hbsu in Escherichia coli and secretion into the periplasm"; Gene, 124, 1993, pp. 99-103.
John Ghrayeb, et al.; "Secretion cloning vectors in Escherichia coli"; The EMBO Journal, 3, 1984; pp. 2437-2442.
Hansen M. Hsiung, et al.; "Use of Bacteriocin Release Protein In E. Coli For Excretion of Human Growth Hormone Into the Culture Medium"; Biotechnology, vol. 7, Mar. 1989; pp. 267-271.
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Bagdasarian et al. Gene. 26 (1983) 273-282, Elsevier "Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors".
Tsung et al Proc. Natl. Acad Sci. USA, vol. 87, pp. 5940-5944, Aug. 1990 "Enhancement of RNA polymerase binding to promoters by a transcriptional activator, OmpR, in Escherichia coli: Its positive and negative effects on transcription".
Gentz et al J. Bacteriology, Oct. 1985, vol. 164, No. 1, pp. 70-77 "Promoters Recognized by Escherichia coli RNA Polymerase Selected by Function: Highly Efficient Promoters from Bacteriophage T5".
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