Promoters and utilization thereof

Details Promoters and utilization thereof Promoters and utilization thereof

2006-10-03

2006-10-03

Collins, Cynthia (Department: 1638)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C546S024000, C435S320100

Reexamination Certificate

active

07115420

ABSTRACT:
The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g.chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

REFERENCES:
patent: 5723754 (1998-03-01), Scott et al.
patent: WO 99/29880 (1999-06-01), None
patent: WO 99/41393 (1999-08-01), None
patent: WO 00/60061 (2000-10-01), None
Kim Y. et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Mol Biol. Jan. 1994;24(1):105-17.
Ha S.B.et al. Cis-acting regulatory elements controlling temporal and organ-specific activity of nopaline synthase promoter. Nucleic Acids Res. Jan. 11, 1989;17(1):215-23.
Anderson W.F. Human gene therapy. Nature. Apr. 30, 1998;392(6679 Suppl):25-30. Review.
Ellis J.G. et al. Does the ocs-element occur as a functional component of the promoters of plant genes? Plant J. Sep. 1993;4(3):433-43.
Namba et al., “Expression of the gene encoding the coat of protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several differenct CMV strains,”Gene(1991), vol. 107, No. 2, pp. 181-188.
P. Meyer and H. Saedler, “Homology-dependent silencing in plants”, Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, pp. 23-48 (1996).
M. A. Matzke and A. J. M Matzke, “How and why do plants inactivate Homologous (Trans)genes?” Plant Physiol. 107, pp. 679-685 (1995).
P. Meyer et al., “Evidence for cytosine methylation of non-symmetrical sequences in transgenic Petunia hybrida”, the EMBO Journal vol. 13, No. 9, pp. 2084-2088 (1994).
A. Razin, “CpG methylation, chromatin structure and gene silencing—a three-way connection”, The EMBO Journal vol. 17, No. 17, pp. 4905-4908 (1998).
T. Hohn et al., “Methylation of coding region alone inhibits gene expression in plant protoplasts”, Proc. Natl. Acad. Sci. USA vol. 93, pp. 8334-8339 (1996).

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