Promoterless foreign gene integrated in Streptococcus thermophil

Details Promoterless foreign gene integrated in Streptococcus thermophil Promoterless foreign gene integrated in Streptococcus thermophil

1993-03-30

1996-02-13

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 671, 4352523, 4352534, C12N 1509, C12N 113, C12P 2100

Patent

active

054910790

ABSTRACT:
A promoterless foreign gene is intergrated into the lac operon of Streptococcus thermophilus by transforming a host strain of Streptococcus thermophilus with a donor plasmid which does not replicate in the host strain. The donor plasmid includes a vector backbone and a sequence containing a foreign gene operably intergrated into at least a part of the lac operon of the host strain, in front of at least a part of the lacZ gene of the lac operon, and the sequence conserves the frame of the genomic lac operon of the host strain. Cointegrate transformants are identified in which the complete donor plasmid is integrated into the geonomic lac operon of the host strain, and an integrant transformant is then selected, the genome of which does not include the vector backbone of the donor plasmid but does include the foreign gene, which is operably integrated in front of the lacZ gene of the conserved genomic lac operon and which is stably maintained and expressed upon selective pressure on expression of the lacZ gene.

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