Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-30
2000-02-15
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 536 221, 536 243, C12Q 168, C12P 1934, C07H 2104
Patent
active
060251336
ABSTRACT:
The present invention features "promoter-sequestered" oligonucleosides and the use of such oligonucleosides to achieve "target-triggered" amplification. A promoter-sequestered oligonucleoside contains a contiguous nucleic acid sequence which forms a stem-loop structure in the absence of a target sequence. The stem-loop structure contains a single-stranded loop region and a double-stranded stem region. The single-stranded loop contains all, or a portion of, an RNA polymerase promoter sequence. The stem is produced from two substantially complementary nucleic acid sequences able to form an intramolecular hybrid. The secondary structure of the stem decreases the accessibility of the loop promoter sequence to form a functional double-stranded promoter.
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Becker Michael M.
Myers Kristi K.
Stull Paul D.
Fisher Carlos A.
Gen-Probe Incorporated
Gritzmacher Christine A.
Heber Sheldon O.
Marschel Ardin H.
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