Promoter sequences

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007210, C435S320100, C435S252300, C435S325000, C435S007200, C536S024100, C424S093100

Reexamination Certificate

active

07045294

ABSTRACT:
The present invention relates an isolated human Site-1 Protease promoter region. The invention also relates to screening methods for agents decreasing the expression of Site-1 protease and thereby being potentially useful for the treatment of medical conditions related to obesity and/or diabetes.

REFERENCES:
patent: WO 00/09677 (2000-02-01), None
Brown et al., “The SREBP Pathway: Regulation of Cholesterol Metabolism by Proteolysis of a Membrane-Bound Transcription Factor,” CELL 89:331-340 (May 2, 1997).
Brown et al., “A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood,” Proc. Natl. Acad. Sci. USA 96:11041-11048 (Sep. 1999).
Horton et al., “Activation of Cholesterol Synthesis in Preference to Fatty Acid Synthesis in Liver and Adipose Tissue of Transgenic Mice Overproducing Sterol Regulatory Element-binding Protein-2,” J. Clin. Invest. 101(11):2331-2339 (Jun. 1998).
Kakuma et al., “Leptin, troglitazone, and the expression of sterol regulatory element binding proteins in liver and pancreatic islets,” PNAS 97(15):8536-8541 (Jul. 18, 2000).
Nagase et al., “Prediction of the Coding Sequences of Unidentified Human Genes. III. The Coding Sequences of 40 New Genes (KIAA0081-KIAA0120) Deduced by Analysis of cDNA Clones from Human Cell Line KG-1,” DNA Research 2:37-43 (1995).
Pai et al., “Differential Stimulation of Cholesterol and Unsaturated Fatty Acid Biosynthesis in Cells Expressing Individual Nuclear Sterol Regulatory Element-binding Proteins,” J. Biological Chemistry 273(40):26138-26148 (1998).
Sakai et al., “Molecular Identification of the Sterol-Regulated Luminal Protease that Cleaves SREBPs and Controls Lipid Composition of Animal Cells,” Molecular Cell 2:505-514 (1998).
Sheng et al., “Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver,” Proc. Natl. Acad. Sci. USA 92:935-938 (Feb. 1995).
Shimano et al., “Overproduction of Cholesterol and Fatty Acids Causes Massive Liver Enlargement in Transgenic Mice Expressing Truncated SREBP-1a,” J. Clin. Invest. 98(7):1575-1584 (Oct. 1996).
Shimomura, “Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy,” Genes & Development 12:3182-3194 (1998).
Xu et al., “Sterol Regulatory Element Binding Protein-1 Expression Is Suppressed by Dietary Polyunsaturated Fatty Acids,” J. Biological Chemistry 274(33):23577-23583 (Aug. 13, 1999).
GenBank™ Accession No. AF078105 (Nov. 19, 1998).

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