Promoter screening vector, streptomyces promoters found therewit

Details Promoter screening vector, streptomyces promoters found therewit Promoter screening vector, streptomyces promoters found therewit

1992-08-07

1994-07-12

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 5, 435 6, 4352351, 4353201, 435 691, 536 221, 536 2372, C07H 2104, C12N 1576, C12N 1574

Patent

active

053289986

ABSTRACT:
The invention relates to a promoter screening vector, to methods for the identification and isolation of Streptomyces promoters using the screening vector, and to the isolated promoters themselves, preferably the pS1 and P14 (SEQ2ID NO: 1) promoters of the S. ghanaensis phase I19.

REFERENCES:
patent: 4918015 (1990-04-01), Wohlleben et al.
Nazarov et al., Biotechnology Letters, vol. 12 (9): 639-644, 1990.
Gurthrie et al., J. Bact. 172 (11): 6189-6193, 1990.
Mervyn J. Bibb and Gary R. Janssen, Unusual Features of Transcription and Translation of Antibiotic Resistance Genes in Antibiotic-Producing Streptomyces, in Fifth International Symposium on the Genetics of Industrial Microorganisms 309-318 (M. Alacevic et al. eds., 1986).
B. K. Leskiw et al., "TTA Codons in Some Genes Prevent Their Expression in a Class of Developmental, Antibiotic-Negative, Streptomyces Mutants," Proc. Natl Acad. Sci. U.S.A., vol. 88, pp. 2461-2465 (1991).
Wolfgang Wohlleben et al., "On the Evolution of Tn21-like Multiresistance Transposons: Sequence Analysis of the Gene (aacCl) for Gentamicin Acetyltransferase-3-I(AAC(3)-I), Another Member of the Tn21-based Expression Cassette," Mol. Gen Genet., vol. 217, pp. 202-208 (1989).

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