Promoter of the gene which codes for the pilinic subunit fim3 of

Details Promoter of the gene which codes for the pilinic subunit fim3 of Promoter of the gene which codes for the pilinic subunit fim3 of

1994-03-16

1995-03-07

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 536 232, 536 237, 536 241, C12N 121, C12N 1511, C12N 1531, C12N 1574

Patent

active

053957644

ABSTRACT:
The isolation and characterization of the promoter regions of the genes which code for the pilinic subunits fim2, fim3 and fimx of Bordetella pertussis, are described, as well as the construction of vectors containing the regions, and microorganisms transformed by the vectors.
The promoter regions, or nucleotide fragments thereof, are particularly useful for the regulable or non-regulable expression of genes which code for a protein of interest in a strain of Bordetella. The transformed Bordetella strains are particularly suitable for the development of an effective anti-pertussis vaccine.

REFERENCES:
patent: 4338299 (1982-07-01), Vesselinova
patent: 4770875 (1988-09-01), Kume et al.
patent: 5059537 (1991-10-01), Pedroni et al.
Suzuki et al. (1986), An Introduction to Genetic Analysis, Third Edition (W. H. Freeman & Co., New York), pp. 315-316.
Mooi et al., FEMS Micro Lett., 66:327-332 (1990).
Willems et al., EMBO J., 9(9):2803-2809 (1990).
Pedroni et al., Mol. Micro., 2(4):539-543 (1988).
Gross et al., Proc. Natl. Acad. Sci. USA 85:3913-3917 (1988).
Livey et al., Mol. Micro. 1:203-209 (1987).
Mooi., et al., Microbial Pathogenesis 2:473-484 (1987).
Shimuzu et al. Infect. Immuno., 36(1):198-201 (1982).
Mooi, Antonie Van Leeuwenhoek, 54(5):465-474 (1988).
Molecular Microbiology, vol. 4, No. 1, 1990, p. 3947; M. J. Walker et al.: "Engineering upstream transcriptional and translational signals of Bordetella pertussis serotype 2 fimbrial subunit protein for efficient expression in Escherichia coli: in vitro autoassembly of the expressed product into filamentous structures".
Weiss et al, "Virulence Factors of Bordetella Pertussis", Ann. Rev. Microbiol., 40:661-686 (1986).
Gross et al, "Pertussis Toxin Promoter Sequences Involved in Modulation", Journal of Bacteriology, 171(7):4026-4030 (1989).
Weiss et al, "Tn5-Induced Mutations Affecting Virulence Factors of Bordetella Pertussis", Infection and Immunity, 42(1):33-41 (1983).
Weiss et al, "Genetic Analysis of Phase Change in Bordetella Pertussis", Infection and Immunity, 43(1):263-269 (1984).
Riboli et al, "Expression of Bordetella Pertussis Fimbrial (fim) genes in Bordetella Bronchiseptica: fixX is Expressed at a Low Level and vir-regulated", Microbial. Pathogenesis., 10:393-403 (1991).
Gentz et al, "Cloning and Analysis of Strong Promoters is Made Possible by the Downstream Placement of a RNA Termination Signal", Proc. Natl. Acad. Sci. USA, 78(8):4936-4940 (1981).
Flock et al, "Expression in Bacillus Subtilis of the Gene for Human Urogastrone Using Synthetic Ribosome Binding Sites", Mol. Gen. Genet., 195:246-251 (1984).

Affiliated with

Also associated with

Rating

Promoter of the gene which codes for the pilinic subunit fim3 of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Promoter of the gene which codes for the pilinic subunit fim3 of, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Promoter of the gene which codes for the pilinic subunit fim3 of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1405591