Promoter for expressing foreign genes in monocotyledonous plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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536 241, 4351723, 4353201, 435419, 935 6, 935 35, 935 64, C12N 500, C12N 1500, A01H 104, C07H 2104

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active

058667938

ABSTRACT:
A DNA fragment for directing the expression of foreign or endogenous genes or RNA in cells of monocot plants. The fragment comprises a sequence corresponding to a first part of a putative type I starch branching enzyme gene (wbeI) present in wheat and a 5'-region upstream of the gene, or a part of the sequence that is effective for increasing the expression of the foreign or endogenous gene in the plant cells. The indicated sequence contains two promoter regions, P1 and P2. A DNA fragment effective to increase expression comprises at least one of the promoter regions, or an effective part. The fragment can be obtained from a genomic library of wheat and can be fused to suitable genes and markers and inserted into suitable vectors for expression in transgenic monocot plants.

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B.ang.ga M., Chibbar R.N. and Kartha K.K. (1995). Molecular cloning and expression analysis of peroxidase genes from wheat. Plant Mol. Biol. 29: 647-662.
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McElroy, D., Zhang, W., Cao, J. and Wu, R. (1990). Isolation of an efficient actin promoter for use in rice transformation. Plant Cell 2: 163-171.
Nehra, N.S., Chibbar, R.N., Leung, N., Caswell, K., Mallard, C., Steinhauer, L., B.ang.ga, M. and Kartha, K.K. (1994). Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. The Plant J. 5(2): 285-297.
MK Morell et al (1977) Plant Physiology 113:201-208.

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