Promoter elements of chimeric genes of .alpha.-tubulin

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 242, 536 236, 4351723, 4353201, 435413, 435414, 435415, 435418, 800DIG9, 800DIG26, 800DIG27, 800DIG43, 800DIG52, 800DIG55, 800DIG56, 800205, C12N 514, C12N 1582, C12N 1509, C12N 1529, A01H 500

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056356188

ABSTRACT:
Regulatory regions from maize .alpha.-tubulin genes are disclosed. In particular, 5' regulatory regions comprising an upstream regulatory ensemble (URE) from maize .alpha.-tubulin 1 and 3 genes is useful in tissue specific expression of heterologous genes in transformed plants. The maize .alpha.-tubulin URE comprises regulatory elements which when operably linked to a promoter and heterologous gene, confer regulated expression in roots, pollen and meristematic tissues. Expression constructs which confer tissue specific expression are also provided.

REFERENCES:
patent: 5086169 (1992-02-01), Mascarenhas
Kim et al. Plant Molecular Biology 24: 105-117 1994 A 20 nucleotide upstream element is essential.
L. Rigau, et al. (1993) "Analysis of a Maize Alpha-Tubulin Gene Promoter by Transient Expression and in Transgenic Tobacco Plants" The Plant Journal 4:6 1043-1060.
L. Montoliu, et al. (1989) "A Tandem of Alpha-Tubulin Genes Preferentially Expressed in Radicular Tissues from Zia Mays" Plant Molecular Biology 14 1-15.
J.L. Carpenter, et al. (1992) "Preferential Expression of an Alpha-Tubulin Gene of Arabidopsis in Pollen" The Plant Cell 4:5 557-571.
L. Montoliu, et al. (1990) "The Tubalpha3 Gene From Zea Mays: Structure and Expression in Dividing Plant Tissues" Gene 94 201-207.
J.L. Carpenter et al. (1993) "Semi-Constitutive Expression of an Arabidipsis Thaliana Alpha-Tubulin Gene" Plant Molecular Biology 21 937-942.

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