Promoter DNA fragment from coryneform bacteria

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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4351723, 4353201, C07H 2104, C12W 1563

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active

056937814

ABSTRACT:
Coryneform bacteria promoter DNA fragments are disclosed having greater promoter strength in Coryneform bacteria cells than the tac promoter obtained by fusing Escherichia coli trp promoter and lac promoter. The promoter function of some of the promoter DNA fragments is controllable by replacing at least one of the culture-medium ingredients with at least one other ingredient. The sizes and nucleotide sequences of such promoter DNA fragments are also disclosed.

REFERENCES:
Santamaria et al. (1987) Gene, vol. 56, pp. 199-208.
Bardonnet et al. (1991) FEMS Microbiology Letters, vol. 84, pp. 97-102.
Takagi et al. (1986) Agricultural & Biological Chemistry, vol. 50, pp. 2597-2603.
Eikmanns et al, A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promotor brobing, Gene 102 (1991) pp. 93-98.
Barak et al, Construction of a promoter-probe shuttle for Escherichia coli and Brevibacteria, Gene, 95 (1990), pp. 133-135.

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