Promoter arrangement for streptomycetes vectors

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 4351723, 43525235, 435320, 435886, 435 70, 935 6, 935 27, 935 41, 935 61, 935 75, 536 27, C12P 2100, C12P 2102, C12N 1500, C12N 120, C07H 1512

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049180070

ABSTRACT:
A tandem arrangement of two promoters which are each active in Streptomyces is provided. The tandem arrangement provides a considerable increase in protein expression.

REFERENCES:
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Old et al., Principles of Gene Manipulation (Studies in Microbiology, vol. 2) (1985) p. 182.
Hopwood et al., Genetic Manipulation of Streptomyces, a Laboratory Manual, the John Innes Foundation, Norwich England, (1985), pp. 292 and 293.
Chemical Abstracts, vol. 103, Abstract #66066c, issued Sep. 1985, Koller, Klaus Peter, 1985, Tendamislat Production, Ger. Offen. DE 3,331,860.
Katz et al., 1983, J. Gen. Microbiology 129:2703-2714.
Biological Abstracts, vol. 65, Abstract #70404, Backman et al. 1978, Maximizing Gene Expression on a Plasmid Using Recombination in vitro, Cell 13(1): 65-72.
Adams et al., 1984, J. Biol. Chem. 259(12): 7399-7403.
Chemical Abstracts vol. 103: Abstract #99720q, issued Sep. 1985, Murooka et al., 1985, Efficient Expression of a Promoter Controlled Gene: Tandem Promoters of Lambda P.sub.R and P.sub.L Functional in Enteric Bacteria, J. Biotechnol. 2(5): 303-316 (Eng.).
Jaurin et al., Gene 28: 83-91 (1984).
European Search Report: EP 86 11 3627.

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