Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-11-07
2006-11-07
Wehbe, Anne M. (Department: 1632)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S252330
Reexamination Certificate
active
07132527
ABSTRACT:
This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
REFERENCES:
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patent: WO 01/12833 (2001-02-01), None
patent: WO 02/086135 (2002-10-01), None
Rene Amore et al., The fermentation of xylose—an analysis of the expression of Bacillus and Actinoplanes xylose isomerase genes in yeast, Appl. Microbiol. Biotechnol., vol. 30:351-357 (1989).
Martin Fussenegger et al., pTRIDENT, a Novel Vector Family for Tricistronic Gene Expression in Mammalian Cells, Biotechnology and Bioengineering, vol. 57(1):1-10, 1998.
A. Delafuente et al., Restriction end-converting vectors with tandem repeated multiple cloning sites, Gene, vol. 139:83-86, 1994.
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Glick, B. R. and J. J. Pasternak, “Molecular Biotechnology Principles and Applications of Recombinant DNA”, 2ndEdition, American Society for Microbiology, Washington, D.C., 1998. (Entire book not attached—Book Cover page, Title Page & Table of Contents Only).
J. W. Meador, III et al., pTRIPLEscript(TM): A Novel Cloning Vector for Generating in Vitro Transcripts from Tandem Promoters for SP6, T7 and T3 RNA Polymerases, Biotechniques, vol. 18(1):152-154, 156-157, 1995.
Kuang-Hsu Amy
Nair Ramesh V.
Payne Mark S.
Picataggio Stephen K.
Soucaille Philippe
E. I. du Pont de Nemours and Company
Hama Joanne
Wehbe Anne M.
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