Proliferation of hepatocyte precursors

Details Proliferation of hepatocyte precursors Proliferation of hepatocyte precursors

1998-07-15

2000-11-14

Kemmerer, Elizabeth

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435347, 435 691, C12N 508, C12N 510

Patent

active

061468894

ABSTRACT:
A composition which comprises an animal cell population which contains immature animal cells. The immature animal cells are characterized by expression of alpha-fetoprotein or lack of essential expression of alpha-fetoprotein and albumin, and at least a portion of said immature animal cells or at least a portion of the progeny of said immature animal cells is capable of differentiating into cells which express albumin. The cell population is cultured under conditions which result in expansion of the cells. Expansion of the cells may be achieved by culturing the cells in the presence of an extracellular matrix and liver stromal cells; and preferably in the presence of growth factors. Such cells may be used for liver transplantation, artificial livers, and for toxicology and pharmacology studies. Such cells may also be genetically engineered to express proteins or polypepetides of interest.

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