Prolactin immunoassay using synthetic peptide

Details Prolactin immunoassay using synthetic peptide Prolactin immunoassay using synthetic peptide

1983-05-03

1986-04-29

Wiseman, Thomas G.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

2601125R, 424 11, 424 85, 424 88, 514 14, 435 4, 435 7, 436542, 436545, 436547, 436804, 436817, 436822, 436823, G01N 3354, G01N 3356, A61K 3702

Patent

active

045857407

ABSTRACT:
A peptide having the formula ##STR1## is synthesized. The peptide is conjugated, e.g., with bis-diazotized benzidine, at its C-terminus to a carrier, such as bovine serum albumin, to form a synthetic antigen useful for inducing antibody production in a host animal. The antiserum obtained from the host animal is free of cross-reactivity with other pituitary substances and thus particularly advantageous for assay purposes. The peptide, whether unlabeled or labeled with radioactive iodine on the tyrosine moiety, has an affinity to antiserum raised against the conjugate similar to the affinity of natural hPRL to the antiserum. The synthetic peptide is used in radioimmunoassays where the labeled peptide competes for the binding sites in the antiserum with unknown concentrations of hPRL in biological samples.

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