Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-01-26
1996-08-27
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 693, 4351723, 435193, 435207, 4352351, 4352402, 4352404, C12N 510, C12N 1509, C12N 701
Patent
active
055500358
ABSTRACT:
A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell.
A method of expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carrier and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized by the RNA polymerase.
REFERENCES:
patent: 5126251 (1992-06-01), Moss et al.
Fuerst Thomas R.
Moss Bernard
Niles Edward G.
Studier F. William
Carter Philip W.
Fleisher Mindy
The Research Foundation of State University of New York
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