Proinsulin derivative and process for producing human insulin

Details Proinsulin derivative and process for producing human insulin Proinsulin derivative and process for producing human insulin

1996-02-13

1999-10-05

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 4353201, 435325, 435243, 536 234, 536 2351, 530303, 530324, C12N 1517, C12N 1563, C12N 121, C07K 1462

Patent

active

059622679

ABSTRACT:
A process for preparing human insulin, which comprises the steps of: preparing a DNA encoding a human proinsulin derivative having the following formula (I); inserting the DNA into a vector to construct an expression vector for the expression of the human proinsulin derivative; transforming a host cell with the expression vector; culturing the resulting transformant under a condition that allows the expression of the human proinsulin derivative; purifying human proinsulin derivative from the culture; and preparing human insulin from the human proinsulin derivative by an enzymatic hydrolysis:

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