Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1995-06-06
1998-06-23
Russel, Jeffrey E.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
435402, 514 21, 530395, 530892, A61K 3839, C07K 1478
Patent
active
057705623
ABSTRACT:
The isolated proteins kalinin and k-laminin are disclosed to provide adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes of human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 410-495 kDa and exists in a cell-associated form (about 495 kDa) and two medium-associated forms (about 460 and 410 kDa, respectively). The BM165 epitope is located on the 165-kDa and 200 kDa subunits. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. The k-laminin adhesion molecule is an isolated heterotrimeric laminin variant that has a molecular weight of about 650 kDa and separates on western blots into first and second fragments that are similar to the B1 and B2 fragments of laminin. K-laminin also includes a third fragment of 190 kDa that is not immunoreactive with monoclonal antibodies 1F5, 11D5 and 4C7, but is immunoreactive with a monoclonal antibody BM165. Kalinin and k-laminin are preferably covalently associated. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.
REFERENCES:
patent: 4829000 (1989-05-01), Kleinman et al.
patent: 4870160 (1989-09-01), Charonis et al.
patent: 5092885 (1992-03-01), Yamada et al.
patent: 5120828 (1992-06-01), Charonis
patent: 5352668 (1994-10-01), Burgeson et al.
patent: 5444158 (1995-08-01), Engrall et al.
ATCC Catalogue of Cell Lines and Hybridomas, 6th ed., 1988, pp. 328, 381.
ATCC Catalogue of Cell Lines and Hybridomas, 7th ed., 1992, pp. 421, 506.
Stites et al, Basic and Clinical Immunology, 7th ed., published 1991 by Appleton & Lange (Norwalk), pp. 423-425.
Protein Purification Methods, Harris et al, published 1989 by IRL Press, pp. 9-10, 57-60, and 62.
Alstadt et al., "Effect of Basement Membrane Entactin on Epidermal Cell Attachment and Growth," J. Invest. Dermatol. 88:55-59 (Jan. 1987).
Bachinger et al., "The Relationship of the Biophysical and Biochemical Characteristics of Type VII Collagen to the Function of Anchoring Fibrils," J. Biol. Chem. 265:10095-10101 (1990).
Boyce et al., "Cultivation, Frozen Storage, and Clonal Growth of Normal Human Epidermal Keratinocytes in Serum-Free Media," J. Tissue Culture Methods 9:83-93 (1985).
Carter et al., "Epiligrin, a New Cell Adhesion Ligand for Integrin .alpha.3.beta.1 in Epithelial Basement Membranes," Cell 65:599-610 (May 17, 1991).
De Luca et al., "Polarized integrin mediates human keratinocyte adhesion to basal lamina," Proc. Natl. Acad. Sci. USA 6888-6892 (Sep. 1990).
Fine, "GB3 Antigen is an Accurate Marker of Only the Herlitz Subset of Junctional Epidermolysis Bullosa," Abstract, Clin. Res. 38(1) (1990).
Fine et al., "19-DEJ-1, A Hemidesmosome-Anchoring Filament Complex-Associated Monoclonal Antibody" Arch Dermatol 125:520-3 (1989).
Fisher, "Skin--The Ultimate Solution for the Burn Wound," New England J. of Medicine 311:466-467 (Aug. 16, 1984).
Gallico et al., "Permanent Coverage of Large Burn Wounds With Autologous Cultured Human Epithelium," The New England J. of Medicine 311:448-451 (Aug. 16, 1984).
Goldberg, "Is the lamina lucida of the basement membrane a fixation artefact?" European J. of Cell. Biol. 42:365-368 (1986).
Hancock, "Cultured keratinocytes and keratinocyte grafts," BMJ 299:1179-1180 (Nov. 11, 1989).
Heaphy et al., "The Human Cutaneous Basement Membrane-Anchoring Fibril Complex: Preparation and Ultrastructure," J. Invest. Dermatol. 68:177-186 (Apr. 1977).
Keene et al., "Kalinin: A Novel Basement Membrane Component Implicated in Keratinocyte Cell Adhesion," Abstract 2231, Basement Membrane Components (2218-2231) p. 401a (Not dated).
Konter et al., "Adhesion molecule mapping in normal human skin," Arch Dermatol Res 281:454-462 (1989).
Lunstrum et al., "Identification of a New Basement Membrane Antigen which Localizes to the Lamina Lucida and Displays a Novel Tissue Distribution," Abstract 61, Cell Attachment to Extracellular Matrix I (56-71) p. 15a (1989).
Lunstrum et al., "Large Complex Globular Domains of Type VII Procollagen Contribute to the Structure of Anchoring Fibrils," J. Biol. Chem. 261:9042-9048 (1986).
Morris et al., "The Tissue Form of Type VII Collagen Is an Antiparallel Dimer," J. Biol. Chem. 261:5638-5644 (1986).
Mortureux et al., "BM-600 Is Present in Normal Amniotic Fluid," Abstract, J. Invest. Dermatol. 95:481 (Oct. 1990).
O'Connor et al., "Grafting of Burns with Cultured Epithelium Prepared from Autologous Epidermal Cells," The Lancet, p. 75 (Jan. 10, 1981).
Phillips, "Cultured Skin Grafts--Past, Present, Future," Arch Dermatol 124:1035-1038 (Jul. 1988).
Rousselle et al., "Identification of a New Basement Membrane Antigen," Abstract in Western Connective Tissue Society Draft Program and Abstracts for Apr. 26-29, 1990, meeting, Santa Cruz, California.
Rousselle et al., "A Novel Basement Membrane Component Implicated in Keratinocyte Cell Adhesion," J. Cell Biol. 114:567-576 (1991).
Sakai et al., "Type VII Collagen Is a Major Structural Component of Anchoring Fibrils," J. Cell Biol. 103:1577-1586 (Oct. 1986).
Sakai et al., "Fibrillin, A New 35-kD Glycoprotein, Is a Component of Extracellular Microfibrils," J. Cell Biol. 103:2499-2509 (Dec. 1986).
Saksela et al., "Basal Lamina Components in Experimentally Induced Skin Blisters," J. Invest. Dermatol. 77:283-286 (1981).
Takashima et al., "Activation of Rabbit Keratinocyte Fibronectin Receptor Function In Vivo During Wound Healing," J. Invest. Dermatol. 86:585-590 (1986).
Thomas et al., "Cultured epithelia from junctional epidermolysis bullosa letalis keratinocytes express the main phenotypic characteristics of the disease," British J. Dermatol. 122:137-145 (1990).
Verrando et al., "The new basement membrane antigen recognized by the monoclonal antibody GB3 is a large size glycoprotein: modulation of its expression by retinoic acid," Biochemica et et Biophysica Acta 942:45-56 (1988).
Verrando et al., "Monoclonal Antibody GB3, a New Probe for the Study of Human Basement Membranes and Hemidesmosomes," Exp. Cell Res. 170:116-128 (1987).
Waikakul et al., "Application of Freeze-Dried Amniotic Membrane: A Control Trial at the Donor Site of Split-Thickness Skin Grafting," Bulletin of the Hospital for Joint Diseases Orthopaedic Institute 50:27-34 (1990).
Woodley et al., "Burn Wounds Resurfaced by Cultured Epidermal Autografts Show Abnormal Reconstitution of Anchoring Fibrils," JAMA 259:2566-2571 (May 6, 1988).
Alitalo et al., "Extracellular Matrix Components Synthesized by Human Amniotic Epithelial Cells in Culture," Cell 19:1053-1062 (Apr. 1980).
Aratani and Kitagawa, "Enhanced Synthesis and Secretion of Type IV Collagen and Entactin during Adipose Conversion of 3T3-L1 Cells and Production of Unorthodox Laminin Complex," J. of Biological Chem. 263:16163-16169 (Nov. 1988).
Davis et al., "Isolation and Characterization of Rat Schwannoma Neurite-promoting Factor: Evidence that the Factor Contains Laminin," The J. of Neuroscience 5:2662-2671 (Oct. 1985).
Donaldson and Mahan, "Epidermal cell migration on laminincoated substrates," Cell Tissue Res. 235:221-224 (1984).
Edgar et al., "Structural Requirements for the Stimulation of Neurite Outgrowth by Two Variants of Laminin and Their Inhibition by Antibodies," The J. of Cell Biology 106:1299-1306 (1988).
Ehrig et al., "Merosin, a tissue-specific basement membrane protein, is a laminin-like protein," Proc. Natl. Acad. Sci. USA 87:3264-3268 (May 1990).
Engel et al., "Shapes, Domain Organizations and Flexibility of Laminin and Fibronectin, Two Multifunctional Proteins of the Extracellular Matrix," J. Mol. Biol. 150:97-120 (1981).
Frenette et al., "Biosynthesis and Secretion of Laminin and Laminin-associated Glycoproteins by Nonmalign
Burgeson Robert E.
Keene Douglas R.
Lunstrum Gregory P.
Marinkovich M. Peter
Rousselle Patricia
Oregon Health & Sciences University
Russel Jeffrey E.
LandOfFree
Products and methods for improving keratinocyte adhesion to the does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Products and methods for improving keratinocyte adhesion to the , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Products and methods for improving keratinocyte adhesion to the will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1394745