Production, purification, cleavage and use of fusion peptides

Details Production, purification, cleavage and use of fusion peptides Production, purification, cleavage and use of fusion peptides

1993-09-27

1997-07-15

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435 712, 4351723, 435233, 4353201, 4352523, 43525233, 530344, 935 10, 935 22, 935 23, 935 24, 935 27, 935 29, 935 38, 935 41, 935 47, 935 56, 935 72, 935 73, C12N 1509, C12N 1563, C12N 1570, C12N 1562

Patent

active

056482442

ABSTRACT:
A method for producing a fusion peptide. A vector is provided with nucleic acid encoding a carrier peptide and at the 3' end of the nucleic acid, a unidirectional restriction endonuclease cleavage site recognized by a restriction endonuclease with the ability to create a non-palindromic 3-base overhang. The vector is cleaved with the restriction endonuclease to produce a cleaved vector. One or more nucleic acids encoding a desired peptide and having at least a 3-base overhang at each end configured and arranged for ligation with the cleaved vector is then ligated to the cleavage site.

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