Production process of recombinant placental growth factor

Details Production process of recombinant placental growth factor Production process of recombinant placental growth factor

2002-02-05

2010-06-29

Saoud, Christine J (Department: 1647)

Drug, bio-affecting and body treating compositions

Lymphokine

C424S200100, C514S002600, C514S008100, C514S012200, C530S399000, C530S412000, C435S069100, C435S071100

Reexamination Certificate

active

07744862

ABSTRACT:
Process for extracting and purifying the recombinant Placental Growth Factor (PLGF) expressed in inducible prokaryotic expression systems comprising the following steps: I) fermentation of the bacterial cells, II) extraction and purification of the inclusion bodies, III) renaturation of the expressed protein, IV) ion-exchange chromatography, V) reverse-phase chromatography.

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