Production of α-keto butyrate

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using bacteria

Reexamination Certificate

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C435S041000, C435S070100, C435S169000, C435S171000

Reexamination Certificate

active

07144715

ABSTRACT:
A process of obtaining α-keto butyrate by bioconversion is disclosed. The molecule is accumulated in the medium and is apt to subsequent processing to food ingredients, such as top-note flavors. A suitable strain is a natural ilv-3 mutantNeurospora crassastrain, which is capable of accumulating α-keto butyrate in the fermentation medium. By adjusting specific medium parameters, up to 8 g/l medium of α-keto butyrate can be accumulated.

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Thu Thuy Pham et al. XP002198174, “Optimal conditions for the formation of sotolon from alpha-ketobutyric acid in the French “Vin Jaune””.Journal Of Agricultural And Food Chemistry, vol. 43, No. 10, pp. 2616-2619, (1995).
R A Larossa et al: XP001059085, “Toxic Accumulation Of Alpha Ketobutyrate Caused By Inhibition Of The Branched-Chain Amino Acid Biosynthetic Enzyme Acetolactate Synthase InSalmonella-Typhimurium”, Journal Of Bacteriology, vol. 169, No. 4, pp. 1372-1378, (1987).
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