Production of weakly active or inactive mutants of alkaline...

Details Production of weakly active or inactive mutants of alkaline... Production of weakly active or inactive mutants of alkaline...

2007-04-10

2007-04-10

Ramirez, Delia M. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S320100, C435S069100, C435S325000, C435S254200, C435S254230, C435S252300, C530S350000, C536S023100, C536S023200

Reexamination Certificate

active

10395790

ABSTRACT:
The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.

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