Production of viruses in tissue culture without use of serum

Details Production of viruses in tissue culture without use of serum Production of viruses in tissue culture without use of serum

1974-11-04

1978-02-07

Rosen, Sam

Textiles: fiber preparation

195 17, 195 18, 424 89, C12B 300, C12K 900, A61K 3912

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040725650

ABSTRACT:
Growth of cells in tissue culture and virus vaccine production in tissue culture in the absence of serum are disclosed. The cells in tissue culture are grown initially in a chemically defined medium containing protamine zinc insulin, and the protamine zinc insulin medium is removed before virus propagation. Supplementation with serum or serum fractions can be eliminated entirely; and improved virus production in a serum free system can be obtained.

REFERENCES:
chansouria et al.-Quarterly Journal of Surgical Sci., vol. 6, (1970), pp. 113-117.
Higuchi-J. Infect. Dis., vol. 112, (1963), pp. 213-220.
Tribble et al.-J. Infect. Dis., vol. 112, (1963), pp. 221-225.
Tribble et al.-Chem. Abst., vol. 59, (1963), p. 9077c.
Griffiths-Chem. Abst., vol. 74, (1971), p. 72200r.
U.S. Dispensatory-25th Edition, (1955), pp. 688 & 689.
Willmer-Cells and Tissues in Culture, vol. 1, (1965), pp. 103-105 & 593-595.

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