Production of viruses in tissue culture without use of serum

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195 17, 195 18, 424 89, C12B 300, C12K 900, A61K 3912

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active

040725650

ABSTRACT:
Growth of cells in tissue culture and virus vaccine production in tissue culture in the absence of serum are disclosed. The cells in tissue culture are grown initially in a chemically defined medium containing protamine zinc insulin, and the protamine zinc insulin medium is removed before virus propagation. Supplementation with serum or serum fractions can be eliminated entirely; and improved virus production in a serum free system can be obtained.

REFERENCES:
chansouria et al.-Quarterly Journal of Surgical Sci., vol. 6, (1970), pp. 113-117.
Higuchi-J. Infect. Dis., vol. 112, (1963), pp. 213-220.
Tribble et al.-J. Infect. Dis., vol. 112, (1963), pp. 221-225.
Tribble et al.-Chem. Abst., vol. 59, (1963), p. 9077c.
Griffiths-Chem. Abst., vol. 74, (1971), p. 72200r.
U.S. Dispensatory-25th Edition, (1955), pp. 688 & 689.
Willmer-Cells and Tissues in Culture, vol. 1, (1965), pp. 103-105 & 593-595.

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