Production of tyrosine hydroxylase positive neurons

Details Production of tyrosine hydroxylase positive neurons Production of tyrosine hydroxylase positive neurons

2005-01-18

2005-01-18

Kemmerer, Elizabeth C. (Department: 1647)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

C514S002600

Reexamination Certificate

active

06844312

ABSTRACT:
The present invention relates to a method of producing neurons that express the enzyme tyrosine hydroxylase (TH) by subjecting neural stem cells to FGF-1, a protein kinase A activator, a protein kinase C activator, and dopamine/L-DOPA. Surprisingly, when forskolin is used as a protein kinase A activator, it requires only low levels of FGF-1 and forskolin to efficiently produce TH positive neurons from fetal or adult neural stem cells. Also provided are compositions used to produce TH positive neurons and the resulting neural cell culture, as well as a method of treating disease or conditions which are associated with dopamine neuron loss or dysfunction.

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