Production of tryptophan by the bacterium Escherichia coli

Details Production of tryptophan by the bacterium Escherichia coli Production of tryptophan by the bacterium Escherichia coli

1996-09-05

1998-05-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 4353201, 435108, 536 231, 536 232, 536 237, C12N 120, C12N 1500, C12P 2106, C07H 2104

Patent

active

057563454

ABSTRACT:
This invention is concerned with the production of L-tryptophan by strains of Escherichia coli.
The invention provides a host and method for growing that host such that plasmids can be stably maintained in the absence of antibiotics, leading to high productivity of tryptophan in fermentation. It offers significant improvements over previous methods and produces tryptophan free from any contamination by tyrosine an phenylalanine thus simplifying downstream processing.
The E. coli strains having productivity for L-tryptophan comprise in their genome a mutant gene encoding a partially defective tryptophanyl-tRNA synthetase introducing a temperature-conditional tryptophan auxotrophy in the host and further mutations on the chromosome which disable the transport systems encoded by aroP, mtr and tnaB and being transformed with a plasmid containing a tryptophan operon encoding anthranilate synthase freed from feedback-inhibition by tryptophan.

REFERENCES:
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Tribe et al. Applied & Environmental Microbiol. 38(2):181, Aug., 1979.
Ito et al. J. Bacteriol. 99(1) : 279, Jul., 1969.
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Hiraga et al. J. Bacteriol. 96(5): 1880, Nov., 1968.
Hirahara et al Applied & Environmental Microbiology 58(8):2633, Aug., 1992.
Derwent Abstract No. 86-329946/50 and JP610247393A dated Nov. 4, 1986.
Derwent Abstract No. 87-296025/42 and JP620208284A dated Sep. 12, 1987.

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