Production of substances with an acetic acid-producing and assim

Details Production of substances with an acetic acid-producing and assim Production of substances with an acetic acid-producing and assim

1988-02-19

1992-08-18

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 711, 435140, 4351723, 435207, 43525233, 435849, 935 22, 935 23, 935 60, 935 73, C12P 2100, C12P 754, C12N 1552, C12N 938

Patent

active

051399385

ABSTRACT:
Cultivation of microorganisms or animal cells or plant cells is carried out to produce high density cultivation, high cell yield and high production of desired metabolite products by monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cells or plant cells to control the acetate concentration to a set value or less. Preferably, an acetic acid-producing bacterium that is inhibited by acetic acid and is capable of assimilating acetic acid is cultured to produce biologically active substances such as enzymes. The bacterium may be a recombinant Escherichia coli and an inducer which acts on a promoter in an expression vector is added to produce the desired metabolite product. The inducer is 3-.beta.- indolylacrylic acid when the promoter is trp-promoter or isopropyl-.beta.-D-thiogalactoside when the promoter is lac-promoter or tacpromoter.

REFERENCES:
patent: 4654215 (1987-03-01), Yamada et al.
Analytica Chimica Acta, vol. 190, 1986; pp. 195-203, Elsevier Science Publishers B. V., Amsterdam, NL; J. Moller et al.: "On-Line High-Performance Liquid Chromatography for Monitoring Fermentation Processes for Penicillin Production".
Patent Abstracts of Japan, vol. 2, No. 72, 31 May 1978, p. 838 C 7 & JP-A-53 29 985 (Kanegafuchi Kagaku Kogyo K.K.) Mar. 20, 1978.

Affiliated with

Also associated with

Rating

Production of substances with an acetic acid-producing and assim does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of substances with an acetic acid-producing and assim, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of substances with an acetic acid-producing and assim will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1248604