Production of sialylated N -glycans in lower eukaryotes

Details Production of sialylated N -glycans in lower eukaryotes Production of sialylated N -glycans in lower eukaryotes

2011-01-04

2011-01-04

Noakes, Suzanne M (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S068100, C435S069100

Reexamination Certificate

active

07863020

ABSTRACT:
The present invention relates to eukaryotic host cells which have been modified to produce sialylated glycoproteins by the heterologous expression of a set of glycosyltransferases, including sialyltransferase and/or trans-sialidase, to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. Novel eukaryotic host cells expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities (such as those involved in sialylation) to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation.

REFERENCES:
patent: 4414329 (1983-11-01), Wegner
patent: 4617274 (1986-10-01), Wegner
patent: 4683293 (1987-07-01), Craig
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 4808537 (1989-02-01), Stroman et al.
patent: 4812405 (1989-03-01), Lair et al.
patent: 4818700 (1989-04-01), Cregg et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4855231 (1989-08-01), Stroman et al.
patent: 4857467 (1989-08-01), Sreekrishna et al.
patent: 4879231 (1989-11-01), Stroman et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4885242 (1989-12-01), Cregg
patent: 4925796 (1990-05-01), Bergh et al.
patent: 4929555 (1990-05-01), Cregg et al.
patent: 4935349 (1990-06-01), McKnight et al.
patent: 5002876 (1991-03-01), Sreekrishna et al.
patent: 5004688 (1991-04-01), Craig et al.
patent: 5032516 (1991-07-01), Cregg
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5047335 (1991-09-01), Paulson et al.
patent: 5122465 (1992-06-01), Cregg et al.
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5166329 (1992-11-01), Cregg
patent: 5324663 (1994-06-01), Lowe
patent: 5595900 (1997-01-01), Lowe
patent: 5602003 (1997-02-01), Pierse et al.
patent: 5707828 (1998-01-01), Sreekrishna et al.
patent: 5766910 (1998-06-01), Fukuda et al.
patent: 5834251 (1998-11-01), Maras et al.
patent: 5849904 (1998-12-01), Gerardy-Schahn et al.
patent: 5854018 (1998-12-01), Hitzemane et al.
patent: 5861293 (1999-01-01), Kojiri et al.
patent: 5910570 (1999-06-01), Elhammer et al.
patent: 5945314 (1999-08-01), Prieto et al.
patent: 5945322 (1999-08-01), Gotschlich
patent: 5955347 (1999-09-01), Lowe
patent: 5955422 (1999-09-01), Lin
patent: 5962294 (1999-10-01), Paulson et al.
patent: 6017743 (2000-01-01), Tsuji et al.
patent: 6040498 (2000-03-01), Stomp et al.
patent: 6096512 (2000-08-01), Elhammer et al.
patent: 6204431 (2001-03-01), Prieto et al.
patent: 6300113 (2001-10-01), Landry
patent: 6333182 (2001-12-01), Coleman et al.
patent: 6783971 (2004-08-01), Coleman et al.
patent: 6858415 (2005-02-01), Coleman et al.
patent: 6949372 (2005-09-01), Betenbaugh et al.
patent: 7029872 (2006-04-01), Gerngross
patent: 7064191 (2006-06-01), Shinkawa et al.
patent: 7214775 (2007-05-01), Hanai et al.
patent: 2002/0137134 (2002-09-01), Gerngross
patent: 2004/0018590 (2004-01-01), Gerngross et al.
patent: 2004/0171826 (2004-09-01), Hamilton
patent: 2004/0230042 (2004-11-01), Hamilton
patent: 2005/0170452 (2005-08-01), Wildt et al.
patent: 2005/0208617 (2005-09-01), Bobrowicz et al.
patent: 2005/0260729 (2005-11-01), Hamilton
patent: 2006/0024292 (2006-02-01), Gerngross et al.
patent: 2006/0024304 (2006-02-01), Gerngross et al.
patent: 2006/0029604 (2006-02-01), Gerngross et al.
patent: 2006/0034828 (2006-02-01), Gerngross et al.
patent: 2006/0034829 (2006-02-01), Gerngross et al.
patent: 2006/0034830 (2006-02-01), Gerngross et al.
patent: 2006/0040353 (2006-02-01), Davidson et al.
patent: 2006/0078963 (2006-04-01), Gerngross
patent: 2006/0148035 (2006-07-01), Gerngross
patent: 2006/0160179 (2006-07-01), Bobrowicz et al.
patent: 2006/0177898 (2006-08-01), Gerngross
patent: 2006/0211085 (2006-09-01), Bobrowicz
patent: 2006/0286637 (2006-12-01), Hamilton
patent: 2007/0105127 (2007-05-01), Gerngross
patent: 0 905 232 (1999-03-01), None
patent: 1 054 062 (2000-11-01), None
patent: 1 211 310 (2002-06-01), None
patent: 1522590 (2005-04-01), None
patent: 1297172 (2005-11-01), None
patent: 8-336387 (1996-12-01), None
patent: WO 96/21038 (1996-07-01), None
patent: WO 98/05768 (1998-02-01), None
patent: WO 99/31224 (1999-06-01), None
patent: WO 99/54342 (1999-10-01), None
patent: WO 00/52135 (2000-09-01), None
patent: WO 00/61739 (2000-10-01), None
patent: WO 01/14522 (2001-03-01), None
patent: WO 01/25406 (2001-04-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/00879 (2002-01-01), None
patent: WO 03/031464 (2003-04-01), None
patent: WO 03/056914 (2003-07-01), None
patent: WO 2004/003194 (2004-01-01), None
patent: WO 2004/063370 (2004-07-01), None
patent: WO 2004/074458 (2004-09-01), None
patent: WO 2004/074461 (2004-09-01), None
patent: WO 2004/074497 (2004-09-01), None
patent: WO 2004/074498 (2004-09-01), None
patent: WO 2004/074499 (2004-09-01), None
patent: WO 2007/028144 (2007-03-01), None
Yamamoto et al., alpha 2,6-Sialyltransferase Gene Transfection into a Human Glioma Cell Line (U373 MG) Results in Decreased Invasivity, J. Neurochem. 68, pp. 2566-2576, 1997.
Kappel et al., Current Opinion in Biotechnology 3:558-553, 1992.
Taft et al., Trends in Genetics 22(12):649-653, 2006.
Linder, Lab. Anim. 30(5):34-39, 2001.
Bilbo et al., Lab. Anim. 30(1):24-29, 2001.
Holschneider et al., Int. J. Dev. Neuroscience 18 :615-618, 2000.
Wood, Comp. Med. 50(1): 12-15, 2000.
Sigmund, Arterioscler. Throm. Vasc. Biol. 20:1425-1429, 2000.
Witkowski et al., Biochemistry, 38, 11643-11650, 1999.
Wishart et al., Journal of Biological Chemistry, vol. 270, No. 45, pp. 26782-26785, 1995.
Kisselev, Structure, vol. 10, pp. 8-9, 2002.
Li et al., Nature Biotech., vol. 24 (2006), pp. 210-215, “Optimization of humanized IgGs in glycoengineered Pichia pastoris”.
Lifely et al., Glycobiology, vol. 5 (1995), pp. 813-822, “Glycosylation and biological activity of CAMPATH-1H expressed in different cell lines . . . ”.
Yamane-Ohnuki et al., Biotech. Bioengin., vol. 87 (2004), pp. 614-622, “Establishment of FUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing . . . ”.
Weikert et al., Nature Biotech , vol. 17 (1999), pp. 1116-1121, “Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins”.
Maras et al., Glycoconjugate J., vol. 16 (1999), pp. 99-107, “Filamentous fungi as production organisms for glycoproteins of bio-medical interest”.
Seffernick et al., J. of Bacteriol., vol. 183 (2001), pp. 2405-2410, “Melamine deaminase and atrazine chlorohydrolase: . . . ”.
Witkowski et al., Biochemistry, vol. 38 (1999), pp. 11643-11650, “Conversion of a beta-ketoacyl synthase to a malonyl decarboxylase . . . ”.
Broun et al., Science, vol. 282 (1998), pp. 1315-1317, “Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids”.
Abeijon et al., “Molecular Cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter fromKluyveromyces lactis,” Proc. Natl. Acad. Sci. USA93:5963-5968 (1996).
Adachi et al., “Mus Musculus Adult Male Testis cDNA, Riken full length enriched library, clone: 4931438M07 product: mannosidase 2, alpha 2, full insert sequence” XP002293645, Database accession No. AK029913 Abstract, Database EMBL, Dec. 21, 2002.
Alani et al., “A Method for Gene Disruption that Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains,” Genetics 116, 541-545, Aug. 1987.
Altman et al., “Insect cells as hosts for the expression of recombinant glycoproteins,”Glycoconj. J.16(2):109-123 (1999).
Altman et al., “Processing of Asparagine-linked Oligosaccharides in Insect Cells: Evidence for Alpha-Mannosidase II,”Glycoconj. J12(2):150-155 (1995).
Alvaino, C.S., Travassos, L.R., et al. (1999) Sialic

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