Production of rotavirus in yeast

Details Production of rotavirus in yeast Production of rotavirus in yeast

1985-07-26

1989-08-01

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435255, 435 68, 435 70, 4351723, 435320, 935 28, 935 29, 935 56, 935 65, 935 69, C12N 1500, C12N 500, C12P 2100

Patent

active

048533335

ABSTRACT:
There is disclosed a DNA transfer vector capable of replication in either a yeast cell or a bacterial cell and having inserted therein a DNA segment coding for human WA rotavirus surface antigen VP7. The transfer vector carrying the foreign DNA is used to transform yeast cells in order to produce the surface antigen VP7 which is capable of eliciting in vivo production of neutralizing antibodies with respect to human WA rotavirus. The surface antigen VP7 so elaborated and its use in a pharmaceutical vaccine composition are also disclosed.

REFERENCES:
Both et al, Proc. Natl. Acad. Sci., vol. 80, pp. 3091-3095, May 1983, "Serotype-Specific Glycoprotein of Simian 11 Rotavirus: Coding Assignment and Gene Sequence".
Bennetzen et al, J. Biol. Chem., vol. 257(6), Mar. 25, 1982, pp. 3018-3025, "The Primary Structure of the Saccharomyces Cerevisiae Gene for Alcohol Dehydrogenase I".
Gunn et al., J. Virol., Jun. 1985, pp. 791-797, vol. 54(3), "Rotavirus Neutralizing Protein VP7: Antigenic Determinants Investigated by Sequence Analysis and Peptide Synthesis".
Kouvelos et al., J. Gen. Virol., vol. 65, 1984, pp. 1211-1214, "Comparison of Bovine, Simian and Human Rotavirus Structural Glycoproteins".
Glass et al, Virology, vol. 141, pp. 292-298, 1985, "Nucleotide Sequence of the Structural Glycoprotein VP7 Gene of Nebraska Calf Diarrhea Virus Rotavirus: Comparison with Homogolous Genes from Four Strains of Human and Animal Rotaviruses".
Richardson et al, J. Virol., Sep. 1984, pp. 860-862, vol. 51(3), "Nucleotide Sequence of the Gene Encoding the Serotype-Specific Antigen of Human (Wa) Rotavirus: Comparison . . . UK Bovine Rotaviruses".
Broach et al, Gene, vol. 8, 1979, pp. 121-133, "Transformation in Yeast: Development of a Hybrid Cloning Vector and Isolation of the CAN 1 Gene".
Kalica et al, Virology, vol. 112, pp. 385-390, 1981, "Genes of Human (Strain Wa) and Bovine (UK) Rotaviruses That Code for Neutralization and Subgroup Antigens".
McKnight et al, Proc. Natl. Acad. Sci., vol. 80, pp. 4412-4416, Jul. 1983, "Selection of Functional cDNAs by Complementation in Yeast".
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