Production of reverse transcriptase

Details Production of reverse transcriptase Production of reverse transcriptase

1982-01-21

1987-05-05

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 68, 435 91, 435 183, 4351723, 435317, 435193, 935 14, 935 29, 935 73, C12N 120, C12N 900, C12N 1500, C12N 100, C12N 910, C12P 2100, C12P 1934

Patent

active

046632905

ABSTRACT:
Methods are described for the production of the .beta.-subunit of reverse transcriptase using recombinant DNA techniques. Methods and compositions are described for cloning and expressing the gene coding for the .beta.-subunit of reverse transcriptase and characterizing and isolating the gene product. The recombinant plasmids constructed herein contain the necessary signals and controls for efficient expression of the reverse transcriptase gene in bacteria and other microorganisms. The isolated reverse transcriptase gene product is a functional, metabolically stable and biologically active protein.

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