Production of recombinant AAV virions

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

Reexamination Certificate

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C435S320100, C435S471000, C435S483000, C536S023100, C536S024100

Reexamination Certificate

active

07927585

ABSTRACT:
Stocks of infectious rAAV are generated using yeast strains, bacterial strains, and bacteriophages engineered to express the required AAV proteins and harboring rAAV vector sequences. Stocks of rAAV virions of all serotypes and pseudotypes can be generated in prokaryotic and eukaryotic cells using the methods described herein.

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Urabe et al., Insect Cells as a Factory to Produce Adeno-Associated Virus Type 2 Vectors, Human Gene Therapy, 13: pp. 1935-1943, 2002.
Lowin et al., Production of Recombinant Parvovirus B19 VP2-Capsids inSaccharomyces cerevisiaefor use in Diagnostics, IX Parvovirus Workshop, Bologna, Italy, 2002, abstract.
Wang et al., Adeno-Associated Virus Type 2 DNA Replication in Vivo: Mutation Analyses of the D Sequence in Viral Inverted Terminal Repeat, JVI, 1997, vol. 71, No. 4, pp. 3077-3082.
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