Production of proteins using homologous recombination

Details Production of proteins using homologous recombination Production of proteins using homologous recombination

1995-06-06

1999-11-09

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 701, 435355, 435357, 435358, 435364, 4353651, 435455, 435463, 435464, 536 241, C12N 1500

Patent

active

059812141

ABSTRACT:
Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.

REFERENCES:
patent: 4634665 (1987-01-01), Axel et al.
patent: 4656134 (1987-04-01), Ringold
patent: 4950599 (1990-08-01), Bertling
patent: 5272071 (1993-12-01), Chappel
Rubnitz et al Mol. Cell. Biol 4:2253,1984.
Kamarck et al PNAS 84: 5350,1987.
Muller et al Eur. J. Biochem 176:485,1988.
Thomas et al Cell 51: 503,1987.
Palmer et al PNAS 84: 1055,1987.
Boshers et al Sequence Species Transcript & Transl. pp. 511-520,1985.
Foecking et al Gene 45: 101,1986.
Nandi et al PNAS 85: 3845,1988.
Frohman et al Cell 56: 145,1989.
Smithies et al Nature 317: 231,1985.
Nelson et al J of Mol. Appl Gen 2(6): 563,1984.
Thompson et al Cell 56: 313,1989.
Anderson Science 226: 401,1984.
Anderson (1984), "Prospects for Human Gene Therapy," Science 226:401-409.
Foecking and Hofstetter, (1986), "Powerful and versatile enhancer-promoter unit for mammalian expression vectors," Gene 45:101-105.
Frohman et al. (1989), "Cut, Paste, and Save: New Approaches to Altering Specific Genes in Mice," Cell 56:145-147.
Janniere et al. (1985), "Stable gene amplification in the chromosome of Bacillus subtilis", Gene 40:47-55.
Johnson et al. (1989), "Targeting of Nonexpressed Genes in Embryonic Stem Cells Via Homologous Recombination", Science 245:1234-1236.
Kamarck et al. (1987), "Carcinoembryonic antigen family: Expression in a mouse L-cell transfectant and characterization of a partial cDNA in bacteriophage .lambda.gt11," PNAS 84:5350-5354.
Kim and Smithies (1988), "Recombinant fragment assay for gene targeting based on the polymerase chain reaction," Nucleic Acids Res. 16:8887-8903.
Larsen et al. (1986), "Repression mediates cell-type-specific expression of the rat growth hormone gene", Proc. Natl. Acad. Sci. U.S.A. 83:8283-8287.
Le Mouellic et al. (1990), "Targeted replacement of the homeobox gene Hox-3.1 by the Escherichia coli lacZ in mouse chimeric embryos", Proc. Natl. Acad. Sci. U.S.A. 87:4712-4716.
Lewin, B. in: Genes, 1983, Oxford University Press, NY, NY, pp. 188-194.
Liskay et al. (1984), "Homologous Recombination between Repeated Chromosomal Sequences in Mouse Cells," Cold Spring Harbor Symp. Quant. Biol. 49:183-189.
Mansour et al. (1988), "Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes," Nature 336:348-352.
Muller et al. (1988), "Enhancer sequences and the regulation of gene transcription," Eur. J. Biochem. 176:485-495.
Murnane and Yezzi (1988), "Association of High Rate of Recombination with Amplification of Dominant Selectable Gene in Human Cells," Somatic Cell and Molecular Genetics 14:273-286.
Nandi et al. (1988), "Regulated expression of genes inserted at the human chromosomal .beta.-globin locus by homologous recombination," Proc. Natl. Acad. Sci. U.S.A. 85:3845-3849.
Nelson et al. (1984), "Metaphase Chromosome Transfer of Introduced Selectable Markers," J. of Mol. Appl. Gen. 2:563-577.
Old and Primrose, In: Studies in Microbiology, vol. 2. "Principles of Gene Manipulation: An Introduction to Genetic Engineering," 2nd Ed., 1981, University of California Press, p. 194.
Palmer et al. (1987), "Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin firoblasts from an adenosine deaminase-deficient human," Proc. Natl. Acad. Sci. U.S.A. 84:1055-1059.
Palmer et al. (1991), "Genetically modified skin fibroblasts persist long after transplantation but gradually inactivate introduced genes," Proc. Natl. Acad. Sci. U.S.A. 88:1330-1334.
Raibaud et al. (1984), "A technique for integrating any DNA fragment into the chromosome of Escherichia coli", Gene 29:231-241.
Rubnitz and Subramani (1984), "The Minimum Amount of Homology Required for Homologous Recombination in Mammalian Cells," Mol. Cell. Biol. 4:2253-2258.
Sambrook et al. (1989), "Molecular Cloning: A Laboratory Manual" (Cold Spring Harbor Press, 1989) pp. 16.3-16.5.
Smithies et al. (1985), "Insertion of DNA sequences into the human chromosomal .beta.-globin locus by homologous recombination", Nature 317:230-234.
Song, et al. (1987), "Accurate modification of a chromosomal plasmid by homologous recombination in human cells," Proc. Natl. Acad. Sci. U.S.A. 84:6820-6824.
Thomas and Capecchi (1987), "Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells," Cell 51:503-512.
Thompson et al., (1989), "Germ Line Transmission and Expression of a Corrected HPRT Gene Produced by Gene Targeting in Embryonic Stem Cells", Cell 56:313-321.
Urlaub and Chasin (1980), "Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity," Proc. Natl. Acad. Sci. U.S.A. 77:4216-4220.
Watson et al., in: Recombinant DNA, A Short Course, 1983, W.H. Freedman & Co., NY, NY, pp. 41, 47.
Weidle et al. (1988), "Amplified expression constructs for human tissue-type plasminogen activator in Chinese hamster ovary cells: instability in the absence of selective pressure," Gene 66:193-203.
Zhu et al. (1986), "Construction of stable laboratory and industrial yeast strains expressing a foreign gene by integrative transformation using a dominant selection system" Gene 50:225-237.
American Type Culture Collection Catalog of Cell Lines and Hybridomas, Sixth Edition, 1988, p. 151.
Athwal et al. (1977), "Serial transfer of a human gene to rodent cells by sequential chromosome-mediated gene transfer," Proc. Natl. Acad. Sci. USA 74:2943-2947.
Baker and Shulman (1988), "Homologous Recombination between Transferred and Chromosomal Immunoglobulin .kappa. Genes," Mol. Cell. Biol. 8:4041-4047.
Capecchi (1989), "The New Mouse Genetics: Altering the Genome by Gene Targeting," Trends in Genetics 5:70-76.
Connors et al. (1988), "DHFR Coamplification of t-PA in DHFR.sup.+ Bovine Endothelial Cells: In Vitro Characterization of the Purified Serine Protease," DNA 7:651-661.
Fuerst et al. (1986), "Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage t7 RNA polymerase," Proc. Natl. Acad. Sci. U.S.A. 83:8122-8126.
Goldfarb et al. (1982), "Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells," Nature 296:404-409.
Kaufman et al. (1985), "Coamplification and Coexpression of Human Tissue-Type Plasminogen Activator and Murine Dihydrofolate Reductase Sequences in Chinese Hamster Ovary Cells, " Mol. Cell. Biol. 5:1750-1759.
Kaufman et al. (1986), "Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells," Proc. Natl. Acad. Sci. U.S.A. 83:3136-3140.
Kaufman, "High Level Production of Proteins in Mammalian Cells," In: Genetic Engineering: Principles and Methods (vol. 9) (Plenum Press 1987).
Klobutcher and Ruddle (1981), "Chromosome Mediated Gene Transfer," Ann. Rev. Biochem. 50:533-534.
Lewis and Srinivasan (1983), "Chromosome-Mediated Gene Transfer of Hydroxyurea Resistance and Amplification of Ribonucleotide Reductase Activity," Mol. Cell Biol. 3:1053-1061.
Lowy et al. (1980) "Isolation of Transforming DNA: Cloning the Hamster aprt Gene," Cell 22:817-823.
Michel et al. (1985), "Expression of Amplified Hepatitis B Virus Surface Antigen Genes in Chinese Hamster Ovary Cells," Biotechnology 3:561-566.
Saxon and Stanbridge (1987), "Transfer and Selective Retention of Single Specific Human Chromosomes via Microcell-Mediated Chromosome Transfer," Meth. Enz. 151:313-325.
Scahill et al. (1983), "Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells," Proc. Natl. Acad. Sci. U.S.A. 80:4654-4658.
Mansour, et al. (1988) Nature 336:348-352 "Disruption of the proto-oncogene int-2 in mouse embryo-derived stem

Affiliated with

Also associated with

Rating

Production of proteins using homologous recombination does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of proteins using homologous recombination, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of proteins using homologous recombination will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1454161