Production of proteins in active forms

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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435 693, 435 694, 435 6951, 435 695, 530408, 530409, 530350, 530399, 530420, 530422, 530423, 530412, 530404, 530405, C07K 1526, C07K 300

Patent

active

049669630

ABSTRACT:
A method for the preparation of proteins in biologically active form including providing a source of protein solubilized from inclusion bodies with a cationic surfactant; providing a weak denaturing agent; and contacting the solubilized protein with the weak denaturant in water in an amount sufficient to allow the protein to remain in a biologically active form.

REFERENCES:
patent: 4731440 (1988-03-01), Bentle et al.
patent: 4797474 (1989-01-01), Patroni et al.
Osterman, Lev A., Methods of Protein and Nucleic Acid Research, vol. 1, Springer-Verlag, New York, 1984.

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