Production of proteins in active forms

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530350, 530399, 530412, 530413, 530414, 530416, 530417, 530422, 435 691, 435 694, 435 6951, 435 695, C07K 312, C07K 328, C07K 1508, C07K 1526

Patent

active

049925318

ABSTRACT:
A method for the recovery of proteins in a solubilized form from host cells including providing a source of host cells incorporating a synthesized or expressed protein; providing a source of at least one cationic surfactant; and treating the host cells with at least one cationic surfactant, in an amount sufficient to effect solubilization of the proteins.

REFERENCES:
patent: 4476049 (1984-10-01), Kung
patent: 4677196 (1987-06-01), Rausch et al.
patent: 4681761 (1987-07-01), Mietzner
patent: 4797474 (1989-01-01), Patroni et al.
Sharma, S. K., 1986, Separation Science and Technology, 21(8):701-726.
Moriyama et al., 1985, Biochimica et Biophysica Acta., 832:135-141.

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