Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-02-01
1996-10-01
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
435 911, 435259, C12N 1500
Patent
active
055610641
ABSTRACT:
The invention relates to a method for producing plasmid DNA, comprising the steps of: (a) lysing cells containing the plasmid DNA to obtain a lysate; (b) treating the lysate by a means for removing insoluble material to obtain a solute; and (c) applying the solute to differential PEG precipitations and chromatography to purify the plasmid DNA. In other embodiments of the invention, the plasmid DNA is produced with GRAS reagents; the plasmid DNA is produced in the absence of enzymes; the plasmid DNA is produced in the absence of organic extractants; the plasmid DNA is produced in the absence of mutagens; the lysing, treating and applying steps are scalable to result in the large scale manufacture of the plasmid DNA; and the lysing, treating and applying steps result in the generation of pharmaceutical grade material.
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Budahazi Gregg
Horn Nancy
Marquet Magda
Meek Jennifer
Martinell James
Vical Incorporated
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