Production of oligosaccharides in transgenic plants

Details Production of oligosaccharides in transgenic plants Production of oligosaccharides in transgenic plants

2008-01-01

2008-01-01

Kallis, Russell P. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters carbohydrate production in the plant

C800S290000, C435S193000, C435S468000, C435S440000, C536S023200, C536S023600

Reexamination Certificate

active

07314973

ABSTRACT:
The invention relates to a method for producing oligosaccharides, comprising selecting a gene which codes for an enzyme which is capable of converting sucrose into an oligosaccharide; linking the gene to suitable transcription-initiation and transcription-termination signals in order to provide an expression construct; transforming a suitable plant cell with the expression construct; regenerating a transgenic plant from the transformed plant cell; culturing the transgenic plant under conditions enabling the expression and activity of the enzyme; and isolating the oligosaccharides from the transgenic plant. The invention further relates to the product obtained by means of the method and to the use thereof, in addition to transgenic plants and parts thereof which are capable of producing oligosaccharides.

REFERENCES:
patent: 4801540 (1989-01-01), Hiatt et al.
patent: 4927811 (1990-05-01), Quarles
patent: 9527878 (1996-01-01), None
patent: 2076647 (1991-08-01), None
patent: 22142308 (1994-03-01), None
patent: 2171313 (1995-03-01), None
patent: 42 27 061 (1994-02-01), None
patent: 43 30 960 (1995-03-01), None
patent: 44 20 223 (1995-05-01), None
patent: 0 133 547 (1985-02-01), None
patent: 0 474 046 (1992-03-01), None
patent: 2072679 (1981-10-01), None
patent: 56 154967 (1981-11-01), None
patent: A-58201980 (1983-11-01), None
patent: A-02163093 (1990-06-01), None
patent: 05504568 (1993-07-01), None
patent: WO89/12386 (1989-12-01), None
patent: 91/13076 (1991-09-01), None
patent: WO91/13076 (1991-09-01), None
patent: 93/02196 (1993-02-01), None
patent: WO93/07159 (1993-04-01), None
patent: WO94/04692 (1994-03-01), None
patent: WO94/14970 (1994-07-01), None
patent: WO94/27617 (1994-12-01), None
patent: WO 95/13389 (1995-05-01), None
patent: WO95/13389 (1995-05-01), None
patent: 96/21023 (1996-07-01), None
Vergauwen et al Plant Physiology 2003, 133:391-401.
Ritsema et al 2004 Plant Molecular Biology 54:853-863.
Turk et al 1997 New Phytology 136:29-38.
Bancal, P., et al., “Fructan Chemical Structure and Sensitivity to an Exohydrolase,”Carbohydrate Research 217:137-151, 1991.
“Bifidus Growth-Stimulating Substances,”Chemical Abstracts 100(17):137377, Apr. 23, 1994.
Chatterton, N.J., et al., “Structure of Fructan Oligomers in Orchardgrass (Dactylis glomerataL.),”J. Plant Physiology 142:552-556, 1993.
Cote, G.L., et al., “Purification and Properties of an Extracellular Levansucrase fromErwinia herbicolaNRRL B-1678,”Carbohydrate Research 190:299-307, 1989.
Ebskamp, M.J.M., et al., “Accumulation of Fructose Polymers in Transgenic Tobacco,”Bio/Technology 12:272-275, Mar. 1994.
Fuchs, A., “Current and Potential Food and Non-Food Applications of Fructans,”Redesigning Crop Products for Biotechnology 19:555-560, 1991.
Geier, G., et al, “Levansucrase as a Virulence Factor in the Etiology of Fireblight,” in W. Zeller (ed.),Communications from the Federal Biological Institute for Agricultural and Forestry Berlin-Dahlem, Symposium on Fireblight, Ladenburg, Germany (Jun. 13-14, 1991), 1992, pp. 78-81. Translation provided.
Hirayama, M., et al., “Production and Characteristics of Fructo-oligosaccharides,”Chemical Abstracts 120(5):52935, Jan. 31, 1994.
John, P., “Fructan Quality and Fructan Synthesis,”Biochemical Society Transactions 19(3):569-572, 1991.
“Microbe belonging to Scopulariopsis generates kestose by culturing microbe in medium containing sucrose and decomposes glucose as by-product,” JP-A-2163093, Jun. 22, 1990. (WPI Database Abstract #9235349.).
Nakano, Y.J., et al., “Mechanism of Streptococcus Mutans Gluco-syltransferases: Hybrid-Enzyme Analysis,”Journal of Bacteriology 174(17):5639-5646, Sep. 1992.
Rastall, R.A., et al. “Synthesis of Oligosaccharides by Reversal of a Fungal B-Glucanase,”Biotechnology Letters 14(5):373-378, May 19, 1992.
Roberfroid, M., “Dietary Fiber, Inulin, and Oligofructose: A Review Comparing Their Physiological Effects,”Crit. Rev. Food Sci. Nutr. 33(2):103-148, 1993. (Medline Database Abstract #93236728.).
Shiroza, T., et al., “Sequence Analysis of theStreptococcus mutansFructosyltransferase Gene and Flanking Regions,”Journal of Bacteriology 170(2):810-816, Feb. 1988.
Simmen, U., et al., “Fructan Synthesis in Excised Barley Leaves,”Plant Physiology 101(2):459-468, Feb. 1993.
Smeekens, S., et al., “Molecular biology of fructan accumulation in plants,”Biochemical Society Transactions 19(3):565-569, 1991.
Steinmetz, M., et al., “The DNA Sequence of the Gene for the SecretedBacillus SubtillisEnzyme Levansucrase and Its Genetic Control Sites,”Mol. Gen. Genet. 200:20-228 (Springer-Verlag 1985).
Van Der Meer, I.M., et al., “Fructan as a New Carbohydrate Sink in Transgenic Potato Plants,”The Plant Cell 6:561-570, Apr. 1994.
Vijn, I., et al., Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybusL.) harbouring onion (Allium cepaL.) Fructan:Fructan 6G-Fructosyltransferase,Plant J. 11(7):387-398, 1997.
Yamamoto, S., et al., “The Mode of Synthesis of Levan by Bacillus Subtilis Levansucrase,”Agricultural and Biological Chemistry 49(2):343-349, Feb. 1985.
Hirayama, M., et al., “Production and Characteristics of Fructo-Oligosaccharides, ” Chemical Abstracts 120:841, 1994.
Luscher, M., et al., “Purification and Characterization on Fructan: Fructan Fructosyltransferase From Jerusalem Artichoke (Helianthus tuberosus L.), ” New Phytology 123:717-724, 1993.
Oakes, J.V., et al., “Production of Cyclodextrins, a Novel Carbohydrate, in the Tubers of Transgenic Potato Plants, ” Bio/Technology 9:982-986, Oct. 1991.
Tokunaga, T., et al., “Influence of Chronic Intake of New Sweetener Frutooligosaccharide (Neosugar) on Growth and Gastrointestinal Function of the Rat, ” Journal of Nutritional Science and Vitaminology 32:111-121, 1986.
Oku, T., “Caloric Evaluation of Reduced-Energy Bulking Sweeteners, ” in D.R. Romsos et al., (eds.), Obesity: Dietary Factors and Control, Japan Scientific Societies Press, Tokyo, 1991, pp. 169-180.

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