Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters carbohydrate production in the plant
Patent
1998-02-05
2000-11-14
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters carbohydrate production in the plant
800288, 800298, 435 697, 435 698, 435101, 435193, 4353201, 435419, 435440, 435468, 536 232, 536 234, 536 236, 536 237, C12N 1529, C12N 1531, C12N 1554, C12N 1582, C12P 1904
Patent
active
061472808
ABSTRACT:
The invention relates to a method for producing oligosaccharides, comprising selecting a gene which codes for an enzyme which is capable of converting sucrose into an oligosaccharide; linking the gene to suitable transcription-initiation and transcription-termination signals in order to provide an expression construct; transforming a suitable plant cell with the expression construct; transforming a suitable plant cell with the expression construct; regenerating a transgenic plant from the transformed plant cell; culturing the transgenic plant under conditions enabling the expression and activity of the enzyme; and isolating the oligosaccharides from the transgenic plant. The invention further relates to the product obtained by means of the method and to the use thereof, in addition to transgenic plants and parts thereof which are capable of producing oligosaccharides.
REFERENCES:
patent: 4801540 (1989-01-01), Hiatt et al.
patent: 4927811 (1990-05-01), Quarles
Vijn et al. Plant J. 11(3): 387-398, 1997.
Roberfroid, M., "Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects" Crit. Rev. Food Sci. Nutr. 33(2):103-148, 1993. (Medline Database Abstract#93236728).
Hirayama, M., et al. "Production and characteristics of fructooligosaccharides," Chemical Abstracts, 120(5):52935, Jan. 31 1994.
Smeekens, S., et al., "Molecular biology of fructan accumulation in plants," Biochemical Society Transactions 19(3):565-569, 1991.
John, P., "Fructan quality and fructan synthesis," Biochemical Society Transactions 19(3):569-572, 1991.
"Bifidus growth-stimulating substances," Chemical Abstracts, 100(17):137377, Apr. 23 1994.
"Microbe belonging to Scopulariopsis generates kestose by culturing microbe in medium containing sucrose and decomposes glucose as by-product," JP-A-2163093, Jun. 22 1990. (WPI Database Abstract#90235349).
Ebskamp, M.J.M. et al., "Accumulation of Fructose Polymers in Transgenic Tobacco", Bio/Technology vol. 12 (Mar. 1994):272-275.
Fuchs, A., "Current and potential food and non-food applications of fructans", Redesigning Crop Products for Biotechnology, vol. 19, pp. 555-560 (1991).
Steinmetz, M. et al., "The DNA sequence of the gene for the secreted Bacillus subtillis enzyme levansucrase and its genetic control sites", Mol Gen Genet (Springer-Verlag, 1985) vol. 200:220-228.
Shiroza, T. et al., Sequence Analysis of the Streptococcus mutans Fructosyltransferase Gene and Flanking Regions, Journal of Bacteriology, vol. 170. No. 2, pp. 810-816 (Feb. 1988).
Cote, G.L. et al., "Purification and Properties of an Extracellular Levansucrase From Erwinia herbicola NRRL B-1678", Carbohydrate Research, 190, pp. 299-307 (1989).
Geier, G. et al., "Levansucrase as a Virulence Factor in the Etiology of Fireblight", Zeller, W. (Ed.) Communications from the Federal Biological Institute for Agricultural and Forestry Berlin-Dahlem, Symposium on Fireblight, Ladenburg, Germany, Jun. 13-14, 1991, vol. 0(0), pp. 78-81 (1992). Translation provided.
Bancal, P. et al., "Fructan chemical structure and sensitivity to an exohydrolase," Carbohydrate Research, 217, pp. 137-151 (1991).
Nakano, Y.J. et al., "Mechanism of Streptococcus Mutans Glucosyltransferases: Hybrid-Enzyme Analysis", Journal of Bacteriology, vol. 174, No. 17, pp. 5639-5646 (Sep., 1992).
Rastall, R.A. et al. "Synthesis of Oligosaccharides by Reversal of a Fungal B-Glucanase", Biotechnology Letters, vol. 14, No. 5, pp. 373-378 (May 19, 1992).
Van Der Meer, I.M. et al., "Fructan as a New Carbohydrate Sink in Transgenic Potato Plants", The Plant Cell, vol. 6, pp. 561-570 (Apr., 1994).
Yamamoto, S. et al. "The Mode of Synthesis of Levan by Bacillus Subtilis Levansucrase", Agricultural and Biological Chemistry, vol. 49 No. 2, pp. 343-349 (Feb., 1985).
Luscher, M. et al, Purification and characterization of fructan: fructan fructosyltransferase from Jerusalem artichoke (Helianthus tuberosus L.) The New Phytologist, vol. 123 No. 4, (Apr. 1993), pp. 717-724.
Simmen, U. et al., "Fructan Synthesis in Excised Barley Leaves", Plant Physiology, vol. 101 No. 2, (Feb. 1993), pp. 459-468.
Chatterton, N.J. et al., "Structure of Fructan Oligomers in Orchardgrass (Dactylis glomerata L.)," J. Plant Physiology, 142, pp. 552-556 (1993).
Ebskamp Michael Johannes Marcus
Geerts Hendrikus Andrianus Maria
Smeekens Josephus Christianus Maria
Weisbeek Petrus Jacobus
Fox David T.
O'Connor Christensen
Stichting Scheikundig Onderzoek in Nederland
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