Production of novel Newcastle disease virus strains from...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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C435S006120, C435S236000, C435S239000

Reexamination Certificate

active

09926431

ABSTRACT:
The present invention concerns cDNAs for making attentuated, infectious Newcastle disease virus (NDV). Another aspect of the invention relates to methods of making the cDNAs. Another aspect of the invention is a vector containing the cDNA optionally linked to an operable promoter. Within the scope of the invention are vaccines comprising the attenuated, infectious NDV. Also disclosed are methods of making the vaccines and methods of using the vaccines to prevent or treat Newcastle disease in an avian host. The present invention also concerns the nucleotide sequences of the entire genome of NDV, the leading region, the trailing region, and the NP region, as well as proteins encoded by these nucleotide sequences.

REFERENCES:
patent: 6146642 (2000-11-01), Garcia-Sastre et al.
patent: 6451323 (2002-09-01), Garcia-Sastre et al.
patent: PCT/NL99/00377 (1999-06-01), None
Millar et al., (Journal of General Virology 1988, 69, 613-620).
Stone, H. D., Avian Diseases vol. 33, pp. 157-162, 1989.
Schijns et al., (Vaccine, vol. 18, pp. 2147-2154, 2000).
Biotech Dictionary: attenuated.
Yusoff, K. et al., “Location of Neutralizing Epitopes on the Fusion Protein of Newcastle Disease Virus Strain Beaudette C”, J. Gen. Virol. 1989, vol. 70, pp. 3105-3109.
Krishnamurthy, S. et al., “Nucleotide Sequences of the Trailer, Nucleocapsid Protein gene and Intergenic regions of Newcastle Disease Virus Strain Beaudette C and Completion of the Entire Genome Sequence”, Journal of General Virology, 1998, vol. 79, pp. 2419-2424.
Schaper, U.M. et al., “Nucleotide Sequence of the Envelope Protein genes of a Highly Virulent, Neurotropic strain of Newcastle Disease Virus”, Virology, 1988, vol. 165, pp. 291-295.
Yusoff, K. et al., “Nucleotide Sequence Analysis of the L Gene of Newcastle Disease Virus: Homologies with Sendai and Vesicular Stomatitis Viruses”, Nucleic Acids Research 1987, vol. 15, No. 10, pp. 3961-3976.
Daskalakis, S. et al., “Nucleotide Sequence of the Phosphoprotein (P) Gene of Newcastle Disease Virus (Strain Beaudette C)”, Nucleic Acids Research, 1992, vol. 20, No. 3, p. 616.

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