Production of non-native bacterial exopolysaccharide in a recomb

Details Production of non-native bacterial exopolysaccharide in a recomb Production of non-native bacterial exopolysaccharide in a recomb

1998-06-11

2000-02-29

Hobbs, Lisa J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435101, 4352523, 435471, 4353201, 435910, 536 232, C12P 1906, C12P 1904, C12N 120, C12N 500, C07H 2104

Patent

active

060308176

ABSTRACT:
A new recombinant bacteria for the production of exopolysaccharides is disclosed as well as a method for making the recombinant bacteria and making an exopolysaccharide from the bacteria by submerged aerobic fermentation of the bacteria utilizing a sugar substrate. The exopolysaccharides obtained from the inventive bacteria exhibit improved, more desirable or different properties from the exopolysaccharide produced by the non-recombinant bacteria from which the recombinant bacteria was derived. In addition, the present invention provides a method of producing bacterial exopolysaccharides by fermentation from sugar substrates that the bacteria which the exopolysaccharides are native to cannot utilize.

REFERENCES:
Pollock et al. (1994) Mechanism of Bacitracin Resistance in Gram-Negative Bacteria That Synthesize Exopolysaccharides. J. Bacteriol. 176 (20): 6229-6237.
Yamazaki et al. (1996) Linkage of Genes Essential for Synthesis of a Polysaccharide Capsule in Sphingomonas Strain S88. J. Bacteriol. 178 (9): 2676-2687.
Papoutsopolou (1994) Genetic Construction of Xanthomonas campestris and Xanthan Gum Production From Whey. Biotechnol. Letts. 16 (12): 1235-1240.

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