Production of neutralizing antibodies by polypeptide VP1 of ente

Drug – bio-affecting and body treating compositions – Lymphokine

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436543, 436547, 435 68, 43524027, 530324, 530806, 530809, 530388, 514 12, A61K 3942

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active

047510838

ABSTRACT:
The production of neutralizing antibodies for poliovirus, or other enteroviruses, are disclosed herein employing the capsid polypeptide VP1. Vaccines can be produced employing this polypeptide in place of killed or live attenuated virus, and the VP1 polypeptide can be employed in assays in place of whole virions. VP1 polypeptide can be produced by isolating it from live virion or can be produced by cloning cDNA sequences coding for this protein in recombinant cDNA vectors.

REFERENCES:
patent: 4544500 (1985-10-01), Bittle et al.
Bachrach, H. L., Moore, D. M., McKercher, P. D. and Polatnichk, J., "Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus", J. Immunol. 115: No. 6, pp. 1636-1641 (1975).
Meloen, R. H., Rowlands, D. J. and Brown, F., "Comparison of the Antibodies Elicited by the Individual Structural Polypeptides of Foot-and-Mouth Disease and Polioviruses", J. GenVirol. 45:761-763 (1979).
Icenogle, J., Gilbert, S. F., Grieves, J., Anderegg, J. and Rueckert, R., "A Neutralizing Monoclonal Antibody Against Poliovirus and Its Reaction with Related Antigens", Virology, 115:211-215 (1981).
Blondel, B., Crainic, R. and Horodniceanu, F., "Le Polypeptide Structural VP1 du Poliovirus Type 1 Induit des Anticorps Neutralisants", C. R. Acad. Sc. Paris, t294 [Serie III-91 (19820)].
Knauert et al., "Monoclonal Antibody Specific for the D Antigenic Form of Poliovirus" in: Abstracts of Ann. Mtg. Amer. Soc. Microbiology, 81, 1981 (US), Abstract T126, p. 258.
Humphreys, D. D. et al., "Monoclonal Antibodies of 4 Different Specificities for Neutralization of Type I Polioviruses", Biological Abstracts, 74, No. 10, 1982.
Osterhaus et al., "Monoclonal Antibodies to Polioviruses: Production of Specific Monoclonal Antibodies to the Sabin Vaccine Strains", Biological Abstracts, 74, 1982.

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