Chemistry: analytical and immunological testing – Involving production or treatment of antibody
Reexamination Certificate
2007-08-21
2007-08-21
Canella, Karen A. (Department: 1643)
Chemistry: analytical and immunological testing
Involving production or treatment of antibody
C436S548000, C530S387100, C530S388100
Reexamination Certificate
active
10014485
ABSTRACT:
A method is provided for producing motif-specific, context-independent antibodies which recognize a plurality of peptides or proteins within a genome that contain the same motif. The method includes the step of immunizing a host with a degenerate peptide library antigen featuring (i) a fixed target motif containing one or more invariant amino acids including at least one modified amino acid, and (ii) a plurality of degenerate amino adds flanking the motif. Motif-specific, context-independent antibodies produced by the disclosed method are also provided. The method encompasses motifs consisting of a single modified amino acid, as well as short motifs comprising multiple invariant amino acids including one or more modified amino acids, such as all or part of kinase consensus substrate motifs, protein-protein binding motifs, or other cell signaling motifs. Methods of using the antibodies, e.g. for genome-wide profiling, are also provided.
REFERENCES:
patent: 5532167 (1996-07-01), Cantley et al.
patent: 5716836 (1998-02-01), Suiko
patent: 544942 (1993-06-01), None
patent: WO98/29452 (1998-07-01), None
Pinilla et al (Peptide Research, 1995, vol. 8, pp. 250-257).
Glenney et al (Journal of Immunological Methods, 1988, vol. 109, pp. 277-285). Cited in co-pending U.S. Appl. No. 10/139,841.
Khachigian, et al.,Journal of Immunological Methods, 140 (1991) 249-258.
U.S. Appl. No. 09/535,364, filed Mar. 24, 2000, Comb et al.
U.S. Appl. No. 09/148,712, filed Sep. 4, 1998, Comb et al.
Karin, Curr. Opin. Cell Biol. 6:415-424 (1994).
Lewis, et al., Adv. Cancer Res. 74:49-139 (1998).
Crowley et al., Cell 77:841-852 (1994).
Fukunaga et al., EMBO 16(8): 1921-1933 (1997).
Stukenberg et al., Curr. Bio. 7:338-348 (1997).
Burbelo et al., Curr. Biol. 5(2): 95-96 (1995).
Yaffe et al., Cell 91:961-971 (1997).
Muslin et al., Cell 84:889-897 (1996).
Struhl, Genes & Dev. 12:599-606 (1998).
Imhof et al., Curr. Biol. 7:689-692 (1997).
Ross et al., Nature 294:654-656 (1981).
Kozma et al., Method. Enzymol. 201:28-43 (1991).
White et al., Method. Enzymol. 201:65-79 (1991).
Kamps, Method. Enzymol. 201:101-111 (1991).
Frackelton et al., Method. Enzymol. 201:79-92 (1991).
Wang, Method. Enzymol. 201:53-65 (1991).
Glenney, Method. Enzymol. 201:92-100 (1991).
Heffetz et al., Method. Enzymol. 201:44-53 (1991).
Czernik et al., Method. Enzymol. 201:264-283 (1991).
Czernik et al., Neuroprot. 6:56-61 (1995).
Paterson & Garrels, Cell biology: A Laboratory Handbook 249-257 (1994), Academic Press.
Fields et al., Pept. Res. 4:95-101 (1991).
Dourtoglou et al., Synthesis 1984:572-574 (1984).
Knorr et al., Tetra. Let. 30:1927-1930 (1989).
Kearney et al., J. Immunol. 123:1548-1550 (1979).
Verhoeven, et al. Science 239:1534-1536 (1988).
Reichmann, et al., Nature (England) 332:323-327 (1988).
Huse, et al., Science 246:1275-1281 (1989).
Knorr, et al., Peptides 1988:37-39 (1989).
Hamaguchi, et al., Molecular & Cellular Biology 8:3035-3042 (1988).
Zha, et al., Cell 87:619-628 (1996).
Westendorf, PNAS, 91:714-718 (1994).
Rosenberg, et al., Journal of Biological Chemistry 268:4499-4503 (1993).
Kushima, et al., Journal of Molecular Neuroscience 8:19-27 (1997).
Yaffe et al, Science 278:1957-1960 (1997).
Cantley, Cell Signaling Technology Inc.'s 2000-2001 Catalogue p. 198.
Nishilawa et al., J. Biol. Chem. 272:952-960 (1990).
Kemp et al., Trends Biochem. Sci. 15:342-346 (1990).
Al-Obeid et al., Biopolymers 47:197-223 (1998).
Yaffe et al., Nature Biotech. 19:348-353 (2001).
Protein Phosphorylation: A Practical Approach, D. Hardie ed., p. 267, IRL Press (1993).
Lichtenberg-Kraag, et al.,Proc. Natl. Acad. Sci. USA, 89: 5384-5388 (Jun. 1992).
Poulter, et al.,J. Biol. Chem. 268: 9636-9644 (1993).
Capra, et al.,Sci. Am. 236: 50-59 (1977).
A Laboratory Manual, Harlow & Lane, pp. 24-27Cold Spring Harbor Laboratory Press (1999).
Immunology, J. Kuby, 3rdEd. pp. 92-96 (1998), Freeman & Co.
Kolle, et al.,FEBS421: 109-114 (1998).
Levine, et al.,Journal of Immunological Methods124: 239 (1989).
Hinson, et al.,Adv. Exp. Med. Biol. 387: 47-55 (1996).
Glenney, et al.,Journal of Immunological Methods109: 277-285 (1988).
Sigma 1998 Catalog pp. 1305 and 1309.
Upstate Biotechnology 1998 Catalog p. 17.
Zymed Laboratories 1996-1997 General Catalog p. 80.
Monoclonal Antibodies, Hybridomas: A New Deminsion in Biological Analyses, Kennett et al. (eds.), Plenum Press (1980).
Antibodies: A Laboratory Manyual, Harlow and Lane (eds.), Cold Spring Harbor Laboratory Press (1988).
Methods in Enzymology, vol. 201, Protein Phosphorylation, Part B Analysis of Protein Phosphorylation, Protein Kinase Inhibitors and Protein Phosphatases, Hunter and Sefton (eds.), Academic Press, Inc. (1991), pp. 3-547.
Songyang, et al., Molecular and Cellular Biology, 16:6486-6493 (1996).
Upstate Biotechnology 2001 Catalog, p. 221.
Cell Signaling Technology, 2000-2001 Catalog, p. 14.
Alessi, et al., FEBS Lett. 399:333-338 (1996).
Franke, et al., Cell 88:435-437, 1997.
Pap, et al., Bio. Chem. 273:19929-19932 (1998).
Datta, et al., Cell 91:231-241 (1997).
Brunet, et al., Cell, 96:857-868 (1999).
Cardone, et al., Science, 282:1318-1321 (1998).
Montminy, Annual Re. Bochem. 66:807-822 (1997).
Songyang, et al., Current Biology 4:973-982 (1994).
Alessi, et al., EMBO J. 15:6541-6551 (1996).
Dalby, et al., J. Biol. Chem. 273:1496-1505 (1998).
Keranen, et al., Curr. Biol., 5:1395-1403 (1995).
Peng, et al., Science, 277:1501-1508 (1997).
Comb Michael J.
Tan Yi
Zhang Hui
Canella Karen A.
Cell Signaling Technology, Inc.
Cullem, Esq. James G.
Warner, Esq. Andrew J.
LandOfFree
Production of motif-specific and context-independent... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of motif-specific and context-independent..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of motif-specific and context-independent... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3826364