Chemistry: molecular biology and microbiology – Condition responsive control process
Patent
1985-08-09
1989-09-12
Warden, Robert J.
Chemistry: molecular biology and microbiology
Condition responsive control process
4352521, 4352534, 435802, C12Q 300, C12R 101, C12R 146
Patent
active
048659690
ABSTRACT:
This invention relates to the production of the microorganisms. The operation is conducted in two stages. The first stage is conducted in the absence of ultrafiltration while the pH of the growth medium is maintained within the range of 6-7 by the addition of a neutralizing agent. Nutrient substratum and dilution water are added during the first stage to maintain the growth rate at a constant level, and the volume of growth medium is permitted to increase. When the amount of growth inhibiting agent produced in the fermentation reaches a predetermined maximum level, the second stage is initiated and the growth rate is maintained in a range of 0.10 to 0.50/hr, which is less than the growth rate in the first stage. By increasing the amount of dilution water the concentration of the growth inhibiting agent is maintained so as to achieve the desired moderate growth rate. The growth inhibiting agent is also continuously removed from the growth medium during the second stage and the volume of the growth medium is maintained substantially constant. The invention may be applied to the production of lactic bacteria, in particular for example in the cheese and wine making industries.
REFERENCES:
patent: 2822319 (1958-02-01), Monod
patent: 3186917 (1965-06-01), Gerhardt et al.
patent: 3418208 (1968-12-01), Coty
patent: 3472765 (1969-10-01), Budd et al.
patent: 3822187 (1974-07-01), Chaffaut et al.
patent: 3911140 (1975-10-01), Osborne et al.
patent: 4167450 (1979-09-01), Chesbro et al.
Stieber et al, Journal of Dairy Science, vol. 63, (1980), pp. 722-730.
Pelczar et al, Microbiology, New York, McGraw-Hill, 1972, pp. 127-128.
Chemical Engineers Handbook, 5th edition, Perry et al (ed.), New York, McGraw-Hill, 1973, pp. 19-83.
Chemical Abstracts, vol. 88, u:11 of Mar. 13, 1978, p. 383; 88:72975m, "Dialysis Continuous Process for Ammonium Lactate Fermentation of Whey: Methematical Model and Computer Simulation" by C. A. Coulman et al.
Chemical Abstracts, vol. 88, u:11 of 3/13/78, p. 383; 72976n, Dialysis Continuous Process for Ammonium Lactate Fermentation of Whey: Experimental Tests, by R. W. Stiepher et al.
Chemical Abstracts, vol. 90, No. 23 of Jun. 4, 1979 ref: 184876c, p. 465 by R. W. Stieber et al, "Dialysis Continuous Process for Ammonium-Lactate Fermentation: Improved Mathematical Model and Use of Deproteinized Whey".
Journal of Dairy Science, vol. 63, No. 5, 1980, by R. W. Stieber et al., pp.722-730, "Production of Lactobacillus Cells by Dialysis Continuous Fermentation of Deproteinized Whey".
Amen Jean
Cabau Michel
Deck Randall E.
Eurozyme S.A.
Warden Robert J.
LandOfFree
Production of microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of microorganisms will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-915805