Production of microbial cells from methanol

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Utilizing media containing lower alkanol

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Details

435253, 435804, 435850, C12N 132, C12N 120, C12R 120

Patent

active

043682715

DESCRIPTION:

BRIEF SUMMARY
FIELD OF TECHNOLOGY

This invention relates to a process for producing microbial cells. More specifically, it relates to a process for producing microbial cells economically in great quantities using novel microorganisms which aerobically assimilate methanol.


BACKGROUND ART

In recent years, much work has been done to develop methods for effectively utilizing microbial cells as feeds, foodstuffs, medicines, industrial materials, etc. To produce microbial cells with industrial advantages, methanol is expected to be used as a major carbon source because there is a good prospect that methanol will be supplied in great quantities and at low costs by the chemical industry. [See, for example, Japanese Laid-Open Patent Publications Nos. 14389/1972 and 15490/1978.]


DISCLOSURE OF THE INVENTION

In view of these circumstances, the present inventors widely searched in nature for microorganisms which would aerobically utilize methanol as a major carbon source and be able to be cultivated with good efficiency, and consequently discovered novel microorganisms, Flavobacterium tosaensis, Pseudomonas wakayamaensis, Flavobacterium methanolicola, Pseudomonas kyotoensis, Pseudomonas aichiensis, and Corynebacterium yamanasiensis. This led to the establishment of a process for producing microbial cells economically in large quantities by utilizing these microorganisms.
Thus, the present invention provides a process for producing microbial cells, which comprises cultivating a bacterium belonging to strains selected from the group consisting of Flavobacterium tosaensis, Pseudomonas wakayamaensis, Flavobacterium methanolicola, Pseudomonas kyotoensis, Pseudomonas aichiensis and Corynebacterium yamanasiensis in a culture medium containing methanol as a major carbon source, and recovering the microbial cells from the culture broth.
The microorganisms used in this invention have been determined to belong to the genus Pseudomonas, the genus Flavobacterium, or the genus Corynebacterium from their bacteriological characteristics. They have been identified as new strains because they differ in various respects from known bacteria belonging to the genus Flavobacterium, known bacteria belonging to the genus Pseudomonas and known bacteria belonging to the genus Corynebacterium, and also because the microorganisms of the genus Flavobacterium used in this invention can utilize methanol whereas known bacteria of the genus Flavobacterium have not been found to assimilate methanol. Thus, these microorganisms have been named Flavoabacterium tosaensis, Pseudomonas wakayamaensis, Flavobacterium methanolicola, Pseudomonas kyotoensis, Pseudomonas aichiensis and Corynebacterium yamanasiensis. Typical bacterial strains belonging to the novel microorganisms used in this invention are Flavobacterium tosaensis DS-1 [FERM-P No. 4058], Flavobacterium methanolicola DS-16 [FERM-P No. 4098], Pseudomonas wakayamaensis DS-25 [FERM-P No. 4100], Pseudomonas kyotoensis DS-22 [FERM-P No. 4099], Pseudomonas aichiensis DS-26 [FERM-P No. 4107], and Corynebacterium yamanasiensis DS-31 [FERM-P No. 4106], all of which are deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan at 5-8-1, Higashi, Inage, Chiba-ken, Japan.
The bacteriological properties of these bacterial strains are shown below.
Cultivated in a nutrient medium and a nutrient agar medium at 37.degree. C. for 3 days. days. The colonies are circular with a diameter of 2-3 mm, and are convex or umbonate with a uniform structure, a smooth surface and an entire edge. Yellowish white or milky white, glistening translucent and mucoid. 37.degree. C. for 3 days. The colonies are circular with a diameter of 2-2.5 mm, and are convex or umbonate with a smooth surface and an entire edge. White or opalescent, glistening, translucent and mucoid. days. The colonies have a smooth surface, and are moderately protruded with a yellowish white or milky white color and a glistening gloss. Translucent and mucoid. days. The colonies have a smooth surface, and are moderately protruded with

REFERENCES:
patent: 3063911 (1962-11-01), Tanaka et al.
patent: 3897303 (1975-07-01), Sherk et al.
patent: 3965985 (1976-06-01), Hitzman
patent: 4048013 (1977-09-01), Wagner et al.

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