Production of mature proteins in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

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536 234, 536 236, 536 231, 536 241, 435 691, 435468, 435204, 435 701, 4353201, 800278, 8003202, 800284, 800295, C12N 1582, C12N 504, C12N 1529, A01H 400

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060667818

ABSTRACT:
A chimeric gene for use in producing a mature protein in secreted form by stably transformed plant cells is disclosed. The gene includes a DNA coding sequence encoding a fusion protein having an (i) N-terminal moiety corresponding to the portion of the rice .alpha.-amylase signal sequence peptide identified by SEQ ID: 1 and, (ii) immediately adjacent the C-terminal amino acid of said portion, a protein moiety corresponding to the protein to be produced. Also disclosed are a fusion protein encoded by the gene, and a method of producing a mature protein in secreted form by plant cells.

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